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《ACTA BOTANICA SINICA》 1999-01
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Actin Is Localized in the Nucleolar Skeleton of Physarum polycephalum

ZENG Xian-Lu;XING Miao;JIAO Ming-Da;WANG Xiao-Guang;TAO Wei:HAO Shui (Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024) (Shenzhen University,Shenzhen 518060)(Department of Biology, Changchun 130032)  
Nucleoli were isolated from the interphase nuclei of Physarum polycephalum Schw. The nucleolarskeleton was obtained after DNA and most of the nucleonal proteins were extracted with DNase I, 0. 25 mol/L(NH4)2SO4 and 2 mol /L NaCl. The nucleolar skeleton appeared as a fibrous network structure composed of fibres about 10 to 30 nm in diameter when observed under the electron microscope. SDS-PAGE analyses revealed about 20 polypeptides in the nucleolar skeleton, including a 43 kD polypeptide which is equivalent toactin in molecular weight. Immunofluorescence observations upon slide preparations of the nucleolar skeletonlabeled with anti-actin antibody showed that the nucleolar skeleton emanated bright fluorescence, indicating theexistence of the antigen. Immunodotting assays further localized actin in the protein prepalations of the nucleolar skeleton. Results of immuno--electron microscopy, with anti-actin antibody and protin A-gold as probes,indicated that gold particles were distributed all over the nucleolus of the interphase nucleus.
【CateGory Index】: Q932
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【References】
Chinese Journal Full-text Database 2 Hits
1 CHEN Ting, HUANG Bai qu (Institute of the Genetics and Cytology, Northeast Normal University, Changchun 130024, China);The progress in the research of the nuclear actin[J];Journal of Northeast Normal University (Natural Science Edition);2001-03
2 SHI Liping, LI Yu (Institute of Mycology, Jilin Agricultural University, Changchun, Jilin 130118, China);Advances of Studies on Myxomycete Biology[J];Journal of Jilin Agricultural University;2003-01
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Jiao Mingda 1\ Zeng Xianlu 1\ Wang Xiaoguang 2\ Hao Shui 1 (Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024) (Department of Biology,Changchun Normal College,Changchun 130032);IMMUNOCYTOCHEMICAL IDENTIFICATION OF TROPOMYOSIN IN NUCLEOLI AND NUCLEOLAR MATRIX OF PHYSARUM POLYCEPHALUM [J];ACTA MICROBIOLOGICA SINICA;1999-05
2 Zhai Zhonghe (College of Life Sciences, Peking University, Beijing 100871);NUCLEAR MATRIX-LAMINA-INTERMEDIATE FILAMENT SYSTEM[J];Bulletin of National Science Foundation of China;1998-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Yu (Jilin Agricultural University, Changchun, Jilin 130118, China);Myxomycetology: A Stretch of Fertile Soil, A Research Realm of Great Inspiration--To Look Forward to the Prospect of World Myxomycete Research from the Standpoint of the First, Second and Third International Congress on the Systematics and[J];Journal of Jilin Agricultural University;2002-01
2 LI Yu (Institute of Mycology, Jilin Agricultural University, Changchun, Jilin 130118,China);Textual Research of "Kwei shi[J];Journal of Jilin Agricultural University;2002-02
3 SHI Liping, LI Yu  (Institute of Mycology,Jilin Agricultural University,Changchun,Jilin 130118,China);Studied on Influence of Nutritional Decoctions and pH on Spore Germination in the Myxomycetes[J];Journal of Jilin Agricultural University;2003-03
4 WANG Xiao-li, LI Yan-shuang, LI Yu (Institute of Mycology, Jilin Agricultural University, Changchun 130118, China);Studies on Several Phaneroplasmodia of Myxomycetes[J];Journal of Jilin Agricultural University;2005-02
5 Wang Qi;et al.(Dept.of Agronomy,Jilin Agricultural University,Changchun 130118);A review of the Trichiales Myxomycetes[J];JOURNAL OF JILIN AGRICULTURAL UNIVERSITY;1994-02
6 Chen Shuanglin et al.(Dept.of Agronomy,Jilin Agricultural University,Changchun 130118);A Preliminary Study on Moist Chamber Culture of Myxomycetes[J];JOURNAL OF JILIN AGRICULTURAL UNIVERSITY;1995-03
7 Wang Qi; et al.(Coll. of Chin. Medi. Materials, Jilin Agri. Univ., Changchun 130118);Two New Varieties of Arcyria[J];JOURNAL OF JILIN AGRICULTURAL UNIVERSITY;1995-04
8 Zeng Xianlu(Institute of Genetics and Cytology, Northeast Normal University Changchun 130024)Zhao Jimin ;Wang Xiaoguang(Department of Biology, Changchun Normal CoHege CWangchun 130032);ELECTRON MICROSCOPY STUDIES ON THE NUCLEAR CYCLE OF PHYSARUM POLYCEPHALUM[J];MYCOSYSTEMA;1997-03
9 Wang Xiaoguang;Zeng Xianlu;Zhao Jimin (Department of Biology and Geography. Changchun Normal College. Changchun 130032) (Institute of Genetics and Cytology,Northeast Normal University, Changchun 130024);MORPHOLOGICAL STUDIES ON THE PROCESS OF NUCLEAR RECONSTRUCTION AFTER MITOSIS IN PHYSARUM POLYCEPHALUM[J];MYCOSYSTEMA;1998-03
10 LI Yu LIU Shu-Yan (Section of Plant Pathology, College of Agronomy, Jilin Agricultural University, Changchun 130118);AMPLIFICATION OF THE LONG FRAGMENT OF LYCOGALA FLAVOFUSCUM SMALL SUBUNIT rDNA USING ONE PAIR OF PRIMERS[J];Mycosystema;2003-01
【Secondary References】
Chinese Journal Full-text Database 8 Hits
1 SHI Li-ping1,LI Yu2(1.Experimental Centre,Shengyang Normal University,Shenyang 110034,China; 2.Mycological Institute,Jilin Agricultural University,Changchun 130118,China);Life cycle of Physarum polycephlum[J];Journal of Northeast Normal University(Natural Science Edition);2010-04
2 XING Lu-jun et al(Hebei Tourism Vocational College,Chengde,Hebei 067000);Chemical Prevention of Myxomycetes Disease in Edible Fungi[J];Journal of Anhui Agricultural Sciences;2011-15
3 SHI Liping, LI Yu  (Institute of Mycology,Jilin Agricultural University,Changchun,Jilin 130118,China);Studied on Influence of Nutritional Decoctions and pH on Spore Germination in the Myxomycetes[J];Journal of Jilin Agricultural University;2003-03
4 SHI Li-PingLI Yu (Biological Department of Shengyang Normal University, Shenyang 110034; Mycological Iinstitute of Jilin Agricultural University, Changchun 130118);LIFE CYCLE OF PHYSARELLA OBLONGA[J];;2004-03
5 SHI Li-Ping LI Yu (Biological Department of Shengyang Normal University, Shenyang 110034; Mycological Institute of Jilin Agricultural University, Changchun 130118);LIFE CYCLE OF PHYSARUM GYROSUM[J];;2005-02
6 SHI Li-Ping1, 2 WANG Sheng-Hou1, 2 LI Yu 3* (1 Biological Department of Shengyang Normal University, Shenyang 110034;2 Laboratory Centre of Shengyang Normal University ,Shenyang 110034;3Mycological Institute of Jilin Agricultural University, Changchun 130118);Life cycle of Didymium iridis[J];Mycosystema;2006-03
7 SHI Li-Ping~1 LI Yu~(2*) (~1Experiment Centre of Shengyang Normal University,Shenyang 110034,China; ~2Mycological Institute of Jilin Agricultural University,Changchun 130118);Life cycle of Physarum tenerum[J];Mycosystema;2007-02
8 SHI Li-Ping1 LI Yu 2* 1Experimental Centre of Shengyang Normal University, Shenyang 110034, China 2Mycological Institute of Jilin Agricultural University, Changchun 130118, China;Life cycle of Physarum compressum[J];Mycosystema;2008-06
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