Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《ACTA BOTANICA SINICA》 1999-01
Add to Favorite Get Latest Update

Organogenesis and Plantlet Regeneration in Vitro of Populus euphratica

GU Rui-Sheng;JIANG Xiang-Ning;GUO zhong-Chen(Experimental Center of Forest Biology, Beijing Forestry University, Beijing 100083)(Institute of Botany of Botany,The Chinese Academy of Sciences, Beijing 100093)  
The research of organogenesis and in vitro plantlet regeneration of Populus euphratica Oliver wascarried out using the tender shoots from mature tree as initial explants and MS medium as the basic medium.The effects of plant growth regulators (PGR) on the regeneration were compared. The results showed that theconcentation of PGR was not strictly required for the organogenesis of the excised olgans and callus, but theratio of BA to NAA was important. Calli could be induced from the excised leaves and stems cultured on themedium with 0.5 mg/L BA and 0. 5 mg/L NAA. The embryonic callus could be multiplied in dark on themedium supplemented with 0. 25 mg/L BA and 0. 5 mg/L NAA. For the adventitious bud regeneration of theleaf and callus, supplement with 0. 5 mg/L BA and 0. 1 mg/L NAA was appropriate, giving a regenerationfrequency of 82.9% and 100%, respectively. The suitable level of BA and NAA for the excised stem's was0. 1 mg/L and 0. 01 mg/L respectively, yielding a regeneration frequency of 83%. Rooting occurred on theMS medium with half strength of macronutrient and addition of 0.015 mg/L NAA, and the rooting rate couldreach up to 86. 2%. The techniques of somatic cell cloning of P. euphratica was established in vitro. Theproblems of deterioration of the subcultured shoots were also discussed.
【Fund】: 国家教委优秀青年教师基金
【CateGory Index】: Q943
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 CHEN Yi-yin,CHEN Yu-zhen(College of Biological Sciences and Biotechnology,Beijing 100083,China);Cold Acclimation-Induced Changes in Freezing Resistance,the Contents of Soluble Protein and Proline and Antioxidant Enzyme Activities in Populus euphratica Calli[J];Shandong Agricultural Sciences;2007-03
2 WU Chun-xia(Key Laboratory of Plant Stress Research,College of Life Science,Shandong Normal University,Jinan,Shandong 250014);Influencing Factors on the Culturing of Plant Suspension Cell[J];Journal of Anhui Agricultural Sciences;2009-01
3 Ding Xia; Chen Xiaoyang; Li Yun; Li Wei.School of Biological Sciences and Biotechnology, Beijing For.Univ., 100083, P.R.China.;Bud regeneration system from leaves of Populus euphratica[J];Journal of Beijing Forestry University;2003-02
4 Zhang Pingdong; Kang Xiangyang; Gao Peng; Han Jin. School of Biological Science and Biotechnology, Beijing For. Univ.,100083,P.R.China.;Comparison of different proliferations of Populus euphratica under in vitro culture.[J];Journal of Beijing Forestry University;2003-06
5 Gao Zhun1 Dai Song-xiang1 Chen Shao-liang1,2? Shen Xin1 Wang Rui-gang1 1College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China 2Key Laboratory of Biological Resources Protection and Utilization in Hubei Province, Hubei Institute for Nationalities, Enshi 445000, P. R. China;Isolation of protoplast from callus of Populus euphratica and H~+ fluxes across plasma membrane under NaCl stress[J];中国林学(英文版);2007-03
6 CHEN Ji-qin~(1), HAN Lie-bao~(1), YANG Chun-qi~(2),LI Xue~(1)(1. Turfgrass Institute,Beijing Forestry University,Beijing 100083,China ;2. Adult Education Department of Ezhou University,Ezhou 436000,China);Effect of different explants on callus induction of perennial ryegrass[J];Grassland and Turf;2005-04
7 Wang Jinmao, Yang Minsheng, Zhen Zhixian, Cao Ying(Agricultural University of Hebei, Baoding 071000, P. R. China); G. Naujoks(Federal Research Centre of Forestry and Forest Products, Waldsieversdorf, Germany);Effect of Exogenous Hormones on Organogenesis and Subculturing of Betula pendula[J];Journal of Northeast Forestry University;2006-04
8 YANG Shu-de~(1,3),CHEN Guo-cang~2,ZHANG Cheng-lie~2,CHEN Jia~1,WANG Xue-chen~1 al Science,China Agricultural University,Beijing 100094,China;2 College of Life is and Environmental Molecular Physiology,Institute of Botany,China Academy of Sciences,Beijing 100093,China);Some difference of capacity of osmotic regulation between lanceolate and broad-ovate leaves in Populus euphratica[J];Acta Botanica Boreali-occidentalia Sinica;2004-09
9 ZHANG Ping-dong,KANG Xiang-yang, Han Jing (Key Lab.For Genetics & Breeding of Forest Trees and Ornamental Plants,MOE.Beijing 100083,P.R.China);Preliminary Studies on Grafting Propagation of Populus Euphratica[J];Journal of Arid Land Resources & Environment;2005-04
10 Liu Maoxiu,Shi Junhui,Wang Xinying,Wang Yonghong(Xinjiang Academy of Forestry,Urumqi 830046,China);Result Analysis on Different Grafting Techniques of Populus euphratica Shoots[J];Protection Forest Science and Technology;2013-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Man-li et al(College of Life Science and Technology,Xinjiang University,Urumqi,Xinjiang 830046);Separation and Content Determination of Three Kinds of Endogenous Hormones in Rhizome of Alhagi sparsifolia[J];Journal of Anhui Agricultural Sciences;2012-14
2 WEI Xiao-min,LI Xian-fang,LI Li-hong,WANG Peng(Department of Biologic Engineering,Zhengzhou Animal Husbandry Engineering College,Zhengzhou,Henan 450011,China);Comparison on the Effect of Two Different Rapid Propagation Methods of Tamarix chinensis[J];Journal of Fujian Forestry Science and Technology;2006-03
3 ZHAO Man-li, YANG Ying-chun, YANG Shi-jie, YUAN Zhen-yan College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;Separation and Measurement of Three Kinds of Endogenous Hormones in Rhizome of Alhagi sparsifolia[J];畜牧与饲料科学(英文版);2012-03
4 ZHANG Yuan-yuan,MA Hui-ling,YU Ling,ZHAO Yuan(College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem,Ministry of Education,Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China);Tissue culture and endogenous hormones change of Poa pratensis[J];Journal of Gansu Agricultural University;2013-02
5 LI Xiao1,2,YU Ming-liang2,MA Rui-juan2,SHEN Zhi-jun2 (1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Horticulture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Micropropagation Technique of Yuhua 2 Peach [Prunus persica(L.) Batsch] in vitro[J];Jiangsu Journal of Agricultural Sciences;2008-03
6 Pei Dong Yuan Lichai (The Research Institute of Forestry, CAF Beijing100091) Gu Ruisheng (Faculty of Science, Lisbon University, Portugal Lisbon 1749-016) Jiang Xiangning(Beijing Forestry University Beijing100083);REGULATION OF JUGLANS REGIA COTYLEDON RHIZOGENESIS[J];Scientia Silvae Sinicae;2003-06
7 GAO Hong-bing,TANG Xiao-jie,MENG Qing-fan(Forestry College of Beihua University,Jilin City 132013,Jilin,China);Effect of High Level of 6-BA on the Endogenous Hormones Level Changes in the Vitrification Seedling of Prunus cerasus[J];Forest Research;2006-04
8 JIA Yanhong1, XIONG Qinge2(1.College of Forestry and Horticulture;2.College of Agriculture, Sichuan Agricultural University, Yaan 625014, Sichuan, China);Advance in Research on Plant Somatic Embryogenesis[J];Journal of Sichuan Agricultural University;2003-01
9 CHENG Lei, ZHOU Gen-yu (College of Life and Environment Sciences, Shanghai Teachers University, Shanghai 200234, China);Tissue Culture and Rapid Propagation of Tamarix chinensis Lour[J];Journal of Shanghai Teachers University;2001-02
10 LI Dai-li,KANG Xiang-yang Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083,China;The Status and Prospect of Exodogenous Hormone and Endogenous Hormone Impact in Plant Callus Tissue Culture[J];Letters in Biotechnology;2007-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Gui-hua, WANG Hong-mei, WANG Lian-xiang (Heze Institute of Agricultural Sciences, Heze 274000, China);Study on tissue culture techniques for peony[J];Shandong Agricultural Sciences;2001-05
2 CHEN Yi-yin,CHEN Yu-zhen(College of Biological Sciences and Biotechnology,Beijing 100083,China);Cold Acclimation-Induced Changes in Freezing Resistance,the Contents of Soluble Protein and Proline and Antioxidant Enzyme Activities in Populus euphratica Calli[J];Shandong Agricultural Sciences;2007-03
3 Cai Jian et al (Key Laboratory of Rice Genetics and Breeding of MOA, AAAS, Hefei 230031);Application of AFLP Molecular Marker in Crop Breeding[J];Journal of Anhui Agricultural Sciences;2002-02
4 LIU Shi-peng et al (College of Life Sciences,Yanan University,Yanan,Shaanxi 716000);Stomatal Distribution and Character Analysis of Leaf Epidermis of Jujube under Drought Stress[J];Journal of Anhui Agricultural Sciences;2006-07
5 HUANG Ya-juan et al (Institute of Environmental Sciences,College of Resource and Environmental Sciences,Lanzhou University,Lanzhou,Gansu 730000);Effects of Atmospheric SO_2 on Carbon Isotope Composition of Plants in Lanzhou City[J];Journal of Anhui Agricultural Sciences;2008-34
6 (Oryza sativa L.)and corn (Zea mays L.) Xiang Taihe,Yang Jianbo and Wu Jiadao (Rice Research Institute ,Anhui Academy of Agricultural Sciences,Hefei 230031);Effective establishment of embryogenic cell suspension cultures of rice[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1996-01
7 WU Zhou-he, WU Chuan-mao, XU Yan (Bioengineering Department, Hubei Polytechnic University, Wuhan 430068, China);Study on Chemical Composition of Essential Oil of Perilla Leaves[J];Amino Acids & Biotic Resources;2003-02
8 SHI Cheng-ying~1,CAI Wei-rong~2,GAN Xu-hua~1,WANG Ben-fan~1,ZHAO Liang-xia~1,TANG Xin-yun~1(1.College of Life Science, Anhui Agricultural University,Hefei 230036;2.Department of Food Enigeering ,Anhui University of Technology and Science,Wuhu 241000);Effects of Six Plant Growth Regulators on the Growth of Spirulina platensis A_9[J];Journal of Anhui Agricultural University;2004-01
9 PAN Lei-qing,TU Kang,JIA Ming-min,LU Zhao-xin (College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095);Effects of O_3 Water Washing on the Freshness Keeping of Celery[J];Journal of Anhui Agricultural University;2004-03
10 SHAO Qing-qin~1,YANG Wen-yu~2,FAN Gao-qiong~2(1.Department of Botany,Anhui Science and Technology University,Fengyang 233100,China;2.Department of Agronomy,Sichuan Agriculture University,Ya'an 625014,China);The Effects of Uniconazole on the Wheat Soluble Sugar and Grain Starch Content under Different Nitrogen Levels[J];Journal of Anhui Science and Technology University;2006-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CHEN Yi-yin,CHEN Yu-zhen(College of Biological Sciences and Biotechnology,Beijing 100083,China);Cold Acclimation-Induced Changes in Freezing Resistance,the Contents of Soluble Protein and Proline and Antioxidant Enzyme Activities in Populus euphratica Calli[J];Shandong Agricultural Sciences;2007-03
2 ZHANG Xiao-yan,XU Kun*(College of Horticulture Science and Engineering,Shandong Agricultural University,Taian 271018,China);Effect of Rootstock-scion Interaction on Antioxidant Enzyme System in Leaves of Grafted Eggplant Seedlings under Low Temperature Stress[J];Shandong Agricultural Sciences;2009-06
3 Lu Lingling1 Yi Kexian2 Xu Xuerong1 SunDequan1 Luo Ping1(1Southern Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Science,Zhanjiang,Guangdong,524091,China;2South China University of Tropical Agriculture,Danzhou,Hainan,571737,China);Study on Transplanting Techniques of Tissue Cultural Shoots of Agave Hybrid NO.11648[J];Anhui Agricultural Science Bulletin;2007-18
4 ZHU Hai-yong et al(School of Environment and Planning,Liaocheng University,Liaocheng,Shandong 252059);Study on the Observation of Flowering Phenophase of Several Common Woody Plants in Liaocheng University[J];Journal of Anhui Agricultural Sciences;2010-02
5 YANG Wei-ran et al(Beijing Forestry University Key Laboratory of Tree Breeding Engineering of China,Key Laboratory of Flower and Tree Genetics and Breeding of the Ministry of Education,Key Laboratory of Tree and Flower Breeding and Biotechnology of State Forestry Administration,Beijing 100083);Research on the Tissue Culture of the Hybrids between Cotton-wood and Aspen and the Establishment of Its Regeneration[J];Journal of Anhui Agricultural Sciences;2010-20
6 WANG Jian et al(Hubei Key Laboratory of Natural Products Research and Development,Biotechnology Research Center,China Three Gorges University,Yichang,Hubei 443002);Study on Konjac Glucomannan Accumulation in Cell Suspension Culture of Amorphophallus konjac[J];Journal of Anhui Agricultural Sciences;2010-27
7 YUE Ning et al(College of Biological Science and Biotechnology,Beijing Forestry University,Beijing 100083);Electrophoresis Analysis on the Expressed Proteins in Heteromorphic Leaves of Populus euphratica Oliv[J];Journal of Anhui Agricultural Sciences;2010-32
8 LIU Yan et al(Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China,Ministry of Education,Southwest Forestry University,Kunming,Yunnan 650224);Research Advance of Poplar Tissue Culture[J];Journal of Anhui Agricultural Sciences;2011-04
9 CHEN Yan et al (College of Life Scince,Agricultural University of Hebei,Baoding,Hebei 071001);Screening and Optimization for Culture Conditions of Cell Suspension of Wheat Lovrin 10[J];Journal of Anhui Agricultural Sciences;2011-05
10 XIONG Huan-ying et al(Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi 330004);Research Progress in the Influencing Factors of Agrobacterium-mediated Genetic Transformation of Plants[J];Journal of Anhui Agricultural Sciences;2012-17
China Proceedings of conference Full-text Database 3 Hits
1 LIU Hui, WANG Ben-jie, YUAN Gui-yan, GUO Rui-chen (Dept. of Clinical Phannacology Qilu Hospital of Shandong University, Jinan 250012, China);Quality control of San Ye Hygienical Inhalants[A];[C];2006
2 Feng Deqin Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China Guo Yaojun Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China;The Latest Application of Two Dimensional Electrophoresis in Agriculture and Forest[A];[C];2006
3 HUANG Fang,HUANG Xiao-lan,WU Hui-qin,DENG Xin,LUO Hui-tai, ZHU Zhi-xin,LIN Xiao-shan (Guangdong Provincial Public Laboratory of Analysis and Testing Technology,China National Analytical Center(Guangzhou),Guangzhou 510070,China);Determination of Betaine in Lycium Barbamm by HPLC-MS[A];[C];2010
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved