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Organogenesis and Plantlet Regeneration in Vitro of Populus euphratica

GU Rui-Sheng;JIANG Xiang-Ning;GUO zhong-Chen(Experimental Center of Forest Biology, Beijing Forestry University, Beijing 100083)(Institute of Botany of Botany,The Chinese Academy of Sciences, Beijing 100093)  
The research of organogenesis and in vitro plantlet regeneration of Populus euphratica Oliver wascarried out using the tender shoots from mature tree as initial explants and MS medium as the basic medium.The effects of plant growth regulators (PGR) on the regeneration were compared. The results showed that theconcentation of PGR was not strictly required for the organogenesis of the excised olgans and callus, but theratio of BA to NAA was important. Calli could be induced from the excised leaves and stems cultured on themedium with 0.5 mg/L BA and 0. 5 mg/L NAA. The embryonic callus could be multiplied in dark on themedium supplemented with 0. 25 mg/L BA and 0. 5 mg/L NAA. For the adventitious bud regeneration of theleaf and callus, supplement with 0. 5 mg/L BA and 0. 1 mg/L NAA was appropriate, giving a regenerationfrequency of 82.9% and 100%, respectively. The suitable level of BA and NAA for the excised stem's was0. 1 mg/L and 0. 01 mg/L respectively, yielding a regeneration frequency of 83%. Rooting occurred on theMS medium with half strength of macronutrient and addition of 0.015 mg/L NAA, and the rooting rate couldreach up to 86. 2%. The techniques of somatic cell cloning of P. euphratica was established in vitro. Theproblems of deterioration of the subcultured shoots were also discussed.
【Fund】: 国家教委优秀青年教师基金
【CateGory Index】: Q943
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