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Molecular Cloning of a Vernalization-related cDNA Clone (Vrc)of Vrc79 in Winter Wheat(Triticum aestivum L.)

ZHAO Da-Zhong;CHONG Kang;WAN Li;XU Ji;TAN Ke-Hui(Research Center for Molecular and evelopmental Biology,Institute of Botany, The Chinese Academy of Sciences, Beijing 100093)(Department of Cell Biology and Genetics, College of Life Sciences, Peking Univers  
Vernalization is an essential factor in the flowering development of cold-required plants. There is akey stage of nucleic acid metabolism in the process of vernalization in winter wheat. To probe into the molecular detendnants of vernalization, a cDNA library presumably enriching vernalization-related genes was prepared by specially expressed mRNAs from winter wheat (Triticum aestivum L. cv. Jingdong No. 1 ) plumules at thekey stage of 21 d vernalization being recovered as cDNAs after subtraction with mRNAs from nonvernalized anddevernalized plumules. One vernalization-related cDNA clone (Vrc ), Vrc79, which was only expressed at thekey stage of 21 d vernalization, but not at other stages of nonvernalization, 4 d vernalization and devernalization, was isolated by differential screening of the library, and shown to be a vernalization-specific clone byNorthem blot. Result of homology search suggested that Vrc79 was a novel gene identified in higher plantwhich was different from the cold-stress-induced genes and might play an important role in the floral induction in vemalization-requiring plants.
【CateGory Index】: Q943.2
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