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Cloning of the Homeotic Gene fbp2 from Petunia hybrida and Its Effects in Tobacco Flower

YU Jing-Juan;GUO Feng-Li;ZHAO De-Gang;FU Yong-FuHAN Yu-Zhen;AO Guang-Ming;MENG Fan-Jing(College of Biology, China Agricultural University, Beijing 100094)  
Messenger RNAs were isolated from the petunia (Petunia hybrida L. ) flower buds and cDNAs were synthesized from reverse transcription. The petunia homeotic gene fbp2 was amplified by PCR usingcDNAs as templates. Sequence analysis indicated that the isolated fragment was composed of 686 bp and theregion flanked by two primers showing 99. 6% homology to the sequence reported by Angenent et al. theebases changed, and MADS domain was conserved. Using petunia flower homeotic gene fbp2 cDNA (yfbp2) astarget gene, the expression vector pBBP2 containing CaMV 355 promoter /yfbp2/nos terminator was constructed and was transformed into Agrobacterium tumefacicns strain LBA4404(pAL44404) by direct transformationmethod. Incubating the leaf explants of tobacco (Nicotiana tabacum L. var. samsum) with LBA4404(pal4404) and selecting in the meditun containing 100 mg/L kanamycin, the regenerated resistant plantswere obtained. Polymerase chain reaction and Southern blotting demonstrated that the target gene was integrat- ed into the genome of tobacco. The flowers of the transgenic tobacco were aberrant resulted in expression offbp2, with petal formed on the stamen.
【CateGory Index】: Q943.2
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