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Establishment of a Highly Efficient Ammonia Secreting Mutant of Synechococcus sp. PCC 7942 and the Glutamine Synthetase Activity, Photosynthesis and Growth in Its Immobilized Cells

QIN Jing-Dong; SHAO Ning; SHI Ding-Ji; XU Xu-Dong; ZHANG Jin-Dong;GUO Ping-Zhong; WANG Wen-Qing; TANG Pei-Song (Instisute of Botany, The Chinese Academy of Sciences, Beijing 100093)(Department of Technical Physics, Peking University, Beijing 100871) (Capi  
A recombinate plasmid pDC-ATGS was constructed, which contained the antisense fragment ofglaA gene from Anabaena sp. PCC 7120 and transformed the unicellular cyanobacterium Synechococcus sp.PCC 7942. The foreign DNA was inserted into the site of glnaA locus of the chromosome through the homologous recombination. By using neomyisin, a highly efficient ammonia secretion mutant was selected. After immobilized, the cells of the mutant within polyurethane (PU) foams, glutamine synthesize (GS) and NH4+ se- cretory activity of GS, and its growth and photosynthesis were measured. It was shown that NH4+ secretion ofthe immobilized mutant was enhanced 156 folds which was much higher than that of free-living cells of the wildtype. The activity of GS was decreased by 73. 6%. Growth of the mutant was the same as that of the wildtype. The activity of photosystem II in the immobilized mutant cells increased by 44% with 77 K fluorescencespectroum measurement.
【CateGory Index】: Q943
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