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《ACTA BOTANICA SINICA》 1999-01
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Characteristics and Crystallization of Nitrogenase MoFe Protein from a nif Z Deleted Stain of Azotobacter vinelandii

WANG Dao-Yong; WANG Yao-Ping ;HUANG Ju-FuWANG Zhi-Ping ;ZHONG Ze-PU;LI Jia-Ge(Institute of Botany ,The Chinese Academy of Sciences, Beijing 100093)(Institute of Biophysics, Tha Chinese Academy of Sciences Beijing 100101)(Institute of Nuclear Agrobiology  
nif Z MoFe protein purified from a nif Z deleted strain of Antobacter vinelandii (DJ194) wasshown to be pure by SDS-Polyacrylamide gel electrophoresis. The protein contained 1. 5 Mo atoms and 15. 9 Featoms per molecule, the ratio of Fe to Mo was lower than that of the MoFe protein purified from the wild typestrain of A. vinelandii; and C2H2, H+ -reduction activity and their ratio (C2h4/H2 (Ar) ) were 16. 6%,21 .7% and 77 .2% of those of the wild type MoFe protein, respechvely. Under a somewhat different condi- tion from that for the crystallization of the wild type MoFe protein dark brown rhombohaedron crystals of nif ZMoFe protein were obtained. It indicated that the deletion of the nif Z resulted in the decrease of number or change in the structure of P-cluster in the mutant MoFe protein, which caused the significant structured and function of change of the protein.
【Fund】: 国家攀登项目;;空间计划资助
【CateGory Index】: Q936
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【References】
Chinese Journal Full-text Database 3 Hits
1 HUANG Ju-Fu ; WANG Dao-Yong; DONG Zhi-Gang; WANG Zhi-Ping; LI Jia-Ge(Institute of Botany, The Chinese Academy of Sciences, Beijing 100093)WANG Yao-Ping(Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101);Relationship Between nifZ and the Synthesis of P-cluster in Nitrogenase MoFe Protein of Azotobacter vinelandii[J];ACTA BOTANICA SINICA;1999-11
2 HUANG Ju-Fu, WANG Dao-Yong, DONG Zhi-Gang, WANG Zhi-Ping, ZHANG Hua-Feng, L*B Yu-Bing, XU Xiang-Ming, ZHAO Ying(Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China);Purification and Characteristics of Mn-containing Nitrogenase Component Ⅰ[J];Acta Botanica Sinica;2001-09
3 HUANG Ju_Fu, DONG Zhi_Gang, ZHANG Hua_Feng *, L Yu_Bing, ZHAO Ying, WANG Zhi_Ping (Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China);Purification and Characterization of Cr-containing Nitrogenase Component Ⅰ[J];植物学报(英文版);2002-03
【Citations】
Chinese Journal Full-text Database 1 Hits
1 HUANG Ju Fu WANG Dao Yong WANG Zhi Ping  LUO Ai Ling GU Shu Rong LI Jia Ge (Institute of Botany,The Chinese Academy of Sciences, Beijing 100093);EFFECT OF PEG AND SALT ON CRYSTALLIZATION OF BACTERIOFERRITIN AND NITROGENASE MoFe PROTEIN FROM AZOTOBACTER VINELANDII [J];ACTA BOTANICA SINICA;1998-09
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 HUANG He-qing1, WU Nan1,2, LIN Qing-mei3, LI Xue-song1,3(1. Department of Biology, School of Life Sciences;2. State Key Laboratory for Physical Chemistry of Solid surfaces;3. Research Center of Environmental Science, Xiamen University, Xiamen 361;STUDIES ON CAPACITY OF ACCEPTING ELECTRONS FROM FASHLIVER FERRITIN WITH ELECTROCHEMICAL TECHNOLOGY[J];Acta Biophysica Sinica;2000-04
2 HUANG He-qing1,2, LIN Qing-mei3, LUO Da-min1, ZHU Bin-lin1(1.MOE Laboratory for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005,China; 2.State Key Laboratory for Physical of Chemistry of Solid Surfaces, X;STUDIES ON CAPACITY OF PICKING UP ELECTRONS ON THE SURFACEOF IRON CORE FORM THE FERRITIN OF FISH LIVER[J];Acta Biophysica Sinica;2001-04
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Chinese Journal Full-text Database 10 Hits
1 WANG Jing Lan 1 , WAN Jing Hong 2 , LUO Ling 2 , XIONG Shao Xiang 3 , WANG Guang Hui 3 , HE Fu Chu 2 , QIAN Xiao Hong 1* ( 1 National Center of Biomedical Analysis, Beijing 100850; 2 Institute of Radiation;Development and Preliminary Application of a Peptide Mass Fingerprinting Technique in Proteome Research[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;2000-04
2 Wang, Fei Lin, Yong-qi(Department of Molecular Biology, Jilin University, Changchun) Institute of Pharmacology and. Toxicology, 27 Taiping road, Beijing;Studies on the Octameric and Tetrameric Molybdenum-iron Proteins of Nitrogenase From Azotobacter Vinelandii[J];;1990-04
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10 Wang Zhi ping, Zhang Hui miao,Huang Ju fu *** , Luo Ai ling and Li Jia ge (Institute of Botany,Academia Sinica,Beijing 100093);THE EFFECT OF UREA ON THE ACTIVITY AND STABILITY OF METALLOCLUSTER OF THE MoFe PROTEIN OF NITROGENASE FROM AZOTOBACTER VINELANDII[J];Acta Botanica Sinica;1996-09
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 HUANG Ju_Fu, DONG Zhi_Gang, ZHANG Hua_Feng *, L Yu_Bing, ZHAO Ying, WANG Zhi_Ping (Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China);Purification and Characterization of Cr-containing Nitrogenase Component Ⅰ[J];植物学报(英文版);2002-03
2 ZHANG Hua-Feng 1*, WANG Yao-Ping 2, L*B Yu-Bing 1, ZHAO Ying 1, DAI Xiao-Hu 2, DONG Zhi-Gang 1, HUANG Ju-Fu 1 ** (1. Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China; 2. Institute of Biophysics, The Chinese Acade;Crystalline Growth of Nitrogenase CrFe Protein[J];植物学报(英文版);2002-04
3 ZHAO Jian-Feng, ZHAO Ying, WANG Zhi-Ping, LU Yu-Bing, QIAN Zhong-Xing, HUANG Ju-Fu ( Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China; Institute of Nuclear Agriculture, Zhejiang University, Hangzhou 310029, China);Purification and Activation In Vitro of MoFe Protein from a nifE Deleted Mutant Strain of Azotobacter vinelandii[J];植物学报(英文版);2003-07
4 ZHAO Ying1, ZHAO Jian-Feng1, L?Yu-Bing1, HUANG Ju-Fu1*, WANG Yao-Ping2,CANG Huai-Xing 2, BI Ru-Chang2*(1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany,The Chinese Academy of Sciences, Beijing 100093, China;2. Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China);Crystal Growth of Nitrogenase CrFe Protein and MnFe Protein in Space[J];植物学报(英文版);2003-09
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