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《Acta Botanica Sinica》 2001-01
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The Structural Transformation of Nucleolar DNA and Its Arrangement in Nucleolus of Allium cepa Cells

TAO Wei 1,2 , ZHANG Li_Yong 2, HUANG Bai_Qu 2, JIAO Ming_Da 2, HE Meng_Yuan 2, HAO Shui 2  [WT6BZ](1. Department of Cell Biology, College of Life Sciences, Peking University, Beijing 100871, China; 2. Institute of Genetics and Cytology, Nor  
By using the DNA specific cytochemical staining method (NAMA_Ur) and conventional electron microscopic technique, the authors examined the configuration of intranucleolar DNA in Allium cepa L. cells and found that nucleolar DNA within the fibrillar center (FC) underwent a structural transformation process from condensed to extended state. The authors' observations also displayed a continuous arrangement process of nucleolar DNA, i.e., the extranucleolar DNA entered FC through the nucleolar organizer region (NOR) channel, then extended to the periphery of FC or to the border between FC and dense fibrillar component (DFC), and distributed along the periphery of FC. Thence, by passing through the NOR channel between FCs, the nucleolar DNA continued to transfer to other FCs and arranged in the same above_mentioned forms.
【Fund】: SupportedbytheNationalNaturalScienceFoundationofChina ( 3 9770 3 67) .
【CateGory Index】: Q942
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