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《Acta Botanica Sinica》 2001-01
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Effect of Extracellular Calmodulin on the Cytosolic Ca~(2+) Concentration in Lily Pollen Grains

SHANG Zhong_Lin 1,2 , MA Li Geng 1, WANG Xue Chen 2, SUN Da Ye 1 [WT6BZ](1. College of Biological Science, Hebei Normal University, Shijiazhuang 050016, China; 2. Key Laboratory of Plant Physiology & Biochemistry, Ministry of Agriculture, Bei  
Using Lilium davidii Duchartre pollen as material, the calcium ion_fluorescence indicator fluo_3AM was loaded successfully into the pollen grains by low temperature loading method. Laser confocal scanning microscopy was used to study the effect of extracellular calmodulin on intracellular calcium. It is found that the purified exogenous calmodulin could elevate the intracellular calcium ion concentration, and the effect was correlated with the concentration of exogenous calmodulin to a certain extent. Cell membrane nonpermeable inhibitor of calmodulin, W 7_agarose, and the anti_serum of calmodulin could decrease the cytosolic calcium level. The results show that the endogenous extracellular calmodulin may play an important role in maintaining and increasing the cytosolic calcium level in pollen grain cell.
【Fund】: 国家和河北省自然科学基金重点!( 3 993 0 2 3 0 )及国家重点基础研究规划项目! (G19990 1170 0 )&&
【CateGory Index】: Q942
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