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《Acta Botanica Sinica》 2001-01
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Characterization of Energy_Transduction in Thermal Pretreated Chloroplast from Spinach

PENG Jiang_Xin 1, WAN Chang_Zhao 2, ZOU Yong_Long 1, CHEN Gen_Yun 1, QIAN Yue_Qin 1, YUAN Qin 2, WANG Guo_Qiang 1 * [WT6BZ](1. Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences, Shanghai 200032, China; 2. Crop Research I  
Characterization of energy_transduction on the chloroplast thylakoid membranes from spinach (Spinacia oleracea L.) after thermal pretreatment was investigated. The related reactions of energy_transduction in chloroplasts were seriously affected by thermal pretreatment. The results were obtained as following: (1) The rate of cyclic photophosphorylation declined when the pretreatment temperature increased in the range of 25 to 45 ℃. (2) The thermal pretreatment led to a decrease of the activity of thylakoid membrane_bounded ATPase. (3) Proton uptake of chloroplasts and the fluorescence quenching of 9_aminoacridine (9_AA) in thylakoid membrane decreased after the thermal pretreatment, but addition of dicyclohexylcarbodiimide (DCCD) could partially restore the fluorescence quenching of 9_AA. (4) Both the rates of fast phase in electrochroism absorption change at 515 nm and the millisecond delayed light emission (ms_DLE) of chloroplast showed a progressive decrease upon raising the temperature of pretreatment. (5) Immunbloting analysis showed that the thermal pretreatment caused the changes of protein content and the electrophoresis mobility of thylakoid membrane_bound ATPase and its α_subunit. (6) If the temperature of pretreatment were higher than 33 ℃, oxygen uptake of PSⅠ _mediated in the samples was rapidly inhibited, but addition of sinapine into the reaction medium could partially restore the ability of oxygen uptake in the samples. These results are briefly discussed in relation to the change of permeability of thylakoid membranes, the dissociation of coupling factor complex as well as accumulation of the radicals in the thylakoid membranes after thermal pretreatment.
【Fund】: SupportedbytheStateKeyBasicResearchandDevelopmentPlanofChina (G19980 10 0 0 ) ;KeyPlanoftheNationalNaturalScienceFoundationofChi
【CateGory Index】: Q945.11
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