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《Acta Botanica Sinica》 2001-01
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Expression of TNF _α Gene in Anabaena sp. PCC 7120 and Purification of Its Product by Affinity Chromatography

SHI Ding_Ji 1, YE Xin 1,2 , ZHONG Hui 3, CAI Yi_Ying 1, SHAO Ning 1, PENG Guo_Hong 4, DONG Xiao_Jun 3, WANG Chun_Mei 1, OUYANG Ye_Xin 1, LI Zhen_Jia 3 [WT6BZ](1. Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China;  
The effects of N (NaNO 3) and C (NaAc) source in medium on the expression of tumor necrosis factor_α ( TNF _α) gene in transgenic Anabaena sp. PCC 7120 were compared. The data showed that N source stabilized the expression of foreign protein and C source altered the synthesis of cell walls. Comparing several methods for breaking the cells, supersonic was able to extract TNF_α better than others. For purification of TNF _α, transgenic Anabaena cells were broken, the extracts were precipitated with ammonia sulfate, and the impure TNF_α was eluted from DEAE ion exchange chromatography. Electrophoresis (PAGE_SDS) showed a single band at 17 kD position.
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