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《Acta Botanica Sinica》 2001-01
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Isolation and Characterization of a Mitochondrial atp6 Gene from Soybean (Glycine max)

HE Chao_Ying 1, WU Xiao_Lei 1, ZHANG Jin_Song 1, GAI Jun_Yi 2, CHEN Shou_Yi 1 * [WT6BZ](1. Plant Biotechnology Laboratory, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China; 2. Soybean Research Institute, Nanjing Agric  
Mitochondrial ATPase (mtATPase) complex plays vital roles in higher plants. It consists of a few subunits. In the present study, a new copy of the mtATPase subunit 6 (EC gene (atp6) was cloned and characterized from Glycine max (L.) Merr., which has the shortest opening reading frame of 223 amino acids in all organisms examined and designated as the atp6 copy3 (atp6_3). PCR amplifications of the atp6_3 from 9 soybean cultivars combined with sequencing analysis suggested its wide occurrence in G. max . RFLP analysis of a RILs population implied that paternal inheritance of the atp6_3 might occur in G. max at undetermined frequency. Under salicylic acid (SA)_treated condition, the expression of the atp6 gene was significantly inhibited. The possible role of this inhibition was discussed.
【Fund】: SupportedbytheNationalKeyBasicResearchSpecialFundsofChina (G19980 10 2 0 8)andNationalHigh_techProgramofChina ( 10 1_0 2_0 2_0 3
【CateGory Index】: Q943
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1 QIU Li-juan1, WANG Chang-ling2, ZHOU Guo-an1, CHEN Shou-yi3, CHANG Ru-zhen1 (1National Key Facility for Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm Resources and Biotechnology, the Ministry of Agriculture, Institute of Crop Science, CAAS, Beijing 100081; 2 Biotechnology College, Shenyang Agricultural University, Shenyang 110161; 3Plant Biotechnology Laboratory, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101);Soybean Molecular Breeding[J];Scientia Agricultura Sinica;2007-11
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