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《Acta Botanica Sinica》 2001-01
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Obtainment of Transgenic Tobacco Harboring phbA , phbB and phbC Genes by Twice Transformation

ZHANG Jing_Yu, YE Liang, LI Li, SONG Yan_Ru  [WT6BZ](Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China)  
To avoid the long time required for conventional sexual crossing, transgenic tobacco (Nicotiana tabacum L.) plants harboring phbA gene (encoding 3_ketothiolase) were used as the target plant for the second transformation mediated by Agrobacterium tumefaciens (Smith et Townsend) Conn LBA4404 containing pZCB which was constructed by linking phbB (encoding acetoacetyl_CoA reductase), phbC (encoding PHB synthase) and ctp sequence to pBIB_HYG under the control of CaMV 35S promoter. The hygromycin resistant transformants were morphologically normal and stable integration of phbB and phbC was confirmed by PCR and PCR_Southern. Moreover, RT_PCR_DNA hybridization analysis showed that 6.67% of the transformed tobacco plants could express both phbB and phbC at transcriptional level.
【Fund】: SupportedbytheState“863”HighTechnologyProgram ( 10 1_0 4_0 3_0 4) ;theNationalNaturalScienceFoundationofChina ( 5 9673 0 0 8)
【CateGory Index】: Q943.2
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