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《Acta Botanica Sinica》 2001-01
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Studies on Petunia hybrida Transformed with Flower_meristem_identity Gene AP1

AN Li_Xin 1, LIU Rong_Wei 2, CHEN Zhang_Liang 1, LI Yi 1 * [WT6BZ](1. The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China; 2. Institute of Tissue Culture,  
Flower_meristem_identity gene AP1 was isolated from Arabidopsis thaliana (L.) Heynh. by RT_PCR. The result of the sequencing showed that only a single nucleotide change was found in this AP1 gene compared with the published sequence, but this changed nucleotide did not effect the primary structure of the protein. Driven by the CaMV 35S promoter, AP1 was stably integrated into the genome of Petunia hybrida Vilm. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated transformation. The intergration of the transgene was identified by PCR and Southern blot analysis. The R 0 generation of two transgenic lines could flower earlier and constantly even in the tissue culture flask, differing from the non_transformed Petunia hybrida .
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【CateGory Index】: Q943.2
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