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《Acta Botanica Sinica》 2001-01
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Agrobacterium _mediated Transformation of Rice with Help of Bombardment

MING Xiao_Tian, YUAN Hua_Yi, WANG Li_Jiang, CHEN Zhang_Liang * [WT6BZ](National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China)  
Oryza sativa L. ssp. japonica cv. Zhonghua 8, which is recalcitrant to infection of Agrobacterium tumefaciens (Smith et Townsend) Conn strain EHA105 with ordinary binary vector pCambia 1301, was transformed through Agrobacterium mediated transformation with help of bombardment. The transformation efficiency can be raised greatly. Single copy of gene insertion in the genome of transgenic rice plants was proved by Southern analysis and the expression of GUS gene was observed. GUS gene and hygromycin_resistant gene show 3∶1 segregation in progenies of the transgenic rice plants.
【CateGory Index】: Q943.2
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