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《Acta Botanica Sinica》 2001-01
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Agrobacterium _mediated Transformation of Rice with Help of Bombardment

MING Xiao_Tian, YUAN Hua_Yi, WANG Li_Jiang, CHEN Zhang_Liang * [WT6BZ](National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China)  
Oryza sativa L. ssp. japonica cv. Zhonghua 8, which is recalcitrant to infection of Agrobacterium tumefaciens (Smith et Townsend) Conn strain EHA105 with ordinary binary vector pCambia 1301, was transformed through Agrobacterium mediated transformation with help of bombardment. The transformation efficiency can be raised greatly. Single copy of gene insertion in the genome of transgenic rice plants was proved by Southern analysis and the expression of GUS gene was observed. GUS gene and hygromycin_resistant gene show 3∶1 segregation in progenies of the transgenic rice plants.
【CateGory Index】: Q943.2
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【References】
Chinese Journal Full-text Database 8 Hits
1 WANG Xiu-qin et al(College of Agronomy,Shandong Agricultural University,Taian,Shandong 271018);Research Progress in Transformation Maize Mediated with Agrobacterium Tumefaciens[J];Journal of Anhui Agricultural Sciences;2006-18
2 GENG Li-zhao,LIU Chuan-liang,LI Fu-guang~* on Genetic Improvement,Ministry of Agriculture,Anyang,Henan 455000,China);Application of the Combination of Agrobacterium-mediated Transformation and Particle Bombardment in Plant Genetic Transformation[J];Acta Botanica Boreali-occidentalia Sinica;2005-01
3 Sun Tingting;Zou Li;Xu Jifei;Yu Yang;Northeast Forestry University;Inner Mongolia University;;Transformation of Trichosanthin Gene to Populus euramericana 108[J];Journal of Northeast Forestry University;2014-09
4 Ma Hui+1 Zhao Kaijun+2 Xu Zhengjin+3(1 Institute of Biotechnology Shenyang Agriculture University Shenyang 110161)(2 Key Laborty of Crop Genetics & Breeding Ministry of Agriculture Institution of Crop Breeding andCultivation Chinese Academy of Agricultural Science Beijing 100081)(3 Agronomy Department Shenyang Agriculture University Shenyang 110161);Recent Study and Actuality of Agrobacterium-mediatedTransformation of Rice[J];Progress In Biotechnology;2003-04
5 Kang Junmei1 Xiong Hengshuo1 Yang Qingchuan1 Sun Yan2(1Institute of Animal Science,CAAS,Beijing 100094;2Institute of Grassland Science,CAU,Beijing 100094);Progress on Genetic Engineering of Plant for Insect Resistance[J];Biotechnology Bulletin;2008-01
6 Kong Qianqian 1 Li Zhihui 1 Wang Qiong 1 Lu Mengzhu 2 Chen Jienan 1(1Central South University of Forestry and Technology,Changsha 410004;2 Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091);Application of Nano-scale Genetic Carriers in Plant Transformation[J];Biotechnology Bulletin;2010-06
7 SHEN Qi, SHEN Zi-long (Biotechnology Center, China Pharmaceutical University,Nanjing 210009 );Transgenic Plants Technology and Its Application for Bioproductions[J];Pharmaceutical Biotechnology;2002-02
8 Jiang Yubao, Yu Yuanjie (College of Agronomy, Shandong Agricultural University, Tai'an 271018);Advance of Agrobacterium on Monocotyledons Plants[J];Chinese Agricultural Science Bulletin;2005-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Lan et al (Key Laboratory of Plant Molecular Breeding of Guangdong Province,College of Agriculture,South China Agricultural University,Guangzhou,Guangdong 510642);Progress in Genetic Transformation Mediated by Agrobacterium tumefacien in Rice[J];Journal of Anhui Agricultural Sciences;2009-30
2 ZHANG Li et al(College of Bioengineering,Henan University of Technology,Zhengzhou,Henan 450001);Research Advances in DNA Methylation of Plant[J];Journal of Anhui Agricultural Sciences;2012-06
3 GUO Zhen-fei,LU Shao-yun (Laboratory for Turfgrass Science,College of Life Science,South China Agricultural University,Guangzhou 510642,China);Application of Genetic Engineering in Turfgrass Breeding[J];Acta Agrestia Sinica;2002-03
4 ZHOU Si-jun, YANG Qing-kai (Agronomy School, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC);Practical requirements and development strategies of plant transformation[J];Journal of Northeast Agricultural University;2000-02
5 Liu Qingfa; Tang Kexuan; Ye Jianming; Hao Zhengrong; He Yiyuan; Shen daleng(Institute of Genetics, Fudan University);Optimization of the Conditions for Wheat Genetic Transfermation Mediated by Agrobacterium Tumefaciens[J];JOURNAL OF FUDAN UNIVERSITY;1998-04
6 TANG Wei and Vanessa Samuels North Carolina State University, Forest Biotechnology Group, Centennial Campus Box 7247, Raleigh, NC 27695~ 7247, USA;Genetic Transformation and its Practical Application in Plants[J];发育与生殖生物学学报(英文版);2001-02
7 Ai Shanshan 1,2 Li Jianrong 1,2 Zeng Xiaofang 1,3 Zhao Degang 1,2,31 Guizhou Key Laboratory of Agro-Bioengineering,Guizhou University,Guiyang,550025;2 Laboratory of Genetic Engineering,College of Life Sciences,Guizhou University,Guiyang,550025;3 Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Guiyang,550025;Establishment of the Tissue Culture and Regeneration System of Guizhou Rice Landrace[J];Molecular Plant Breeding;2012-04
8 ZHANG Ming-zhou~(1,2) CUI Hai-rui~1 SHU Qing-yao~1 XIA Ying-wu~1(1.Institute of Nuclear Agricultural Sciences,Zhejiang University,Hangzhou, Zhejiang,310029;2.College of Life Science,China Jiliang University,Hangzou,Zhejiang,310018);REGENERATION SYSTEM AND INFLUENCING FACTORS FOR GENETIC TRANSFORMATION OF SORGHUM SHOOT APEX[J];Journal of Nuclear Agricultural Sciences;2006-01
9 CHEN Caiyan 1,2* ,XIAO Han 1,2* ,ZHANG Wenli 1,2 ,WANG Aiju 1,2 ,XIA Zhihui 1 ,LI Xiaobing 1 , ZHAI Wenxue 1 ,CHENG Zhukuan 1 &ZHU Lihuang 1 1.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Develop- mental Biology,Chinese Academy of Sciences,Beijing 100101,China; 2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China;Adapting rice anther culture to gene transformation and RNA interference[J];中国科学(C辑:生命科学)(英文版);2006-05
10 ZHENG Guang-yu (College of Life Sciences, Beijing Normal University, Beijing 100875, China);Application of Soil Agrobacteria in the Plant Genetic Engineering[J];Journal of Kashgar Teachers College;2004-03
China Proceedings of conference Full-text Database 2 Hits
1 ZHAO Qi~1 YANG Qi-Zhi~1 QU Bai-Yan~2 PAN Ning~1 WANG Zhen-Lian~1 PAN Guang-Tang~2 (~1Capital Normal University, Beijing, 100037; ~2Sichuan Agricultrual University, Yaan, 625014);Expression of thaumatin gene in normal maize embryo[A];[C];2004
2 ZHUO,Chunliu,CAI Jiongliang,FANG Junyang,ZHOU Ming,GUO Zhenfei (Biotechnology Laboratory for Turfgrass and Forages,Department of Plant Science,College of Life Sciences,South China Agricultural University,Guangzhou 510642:China.);Early light induced protein(ELIP) gene from Medicago falcata confers resistance to abiotic stresses[A];[C];2010
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Liu Jinyuan, Shi Xiangyu, Chen Xiao, Zeng Zhaohai,Zhou Zhuhua & Chen Jianai(Institute of Atomic Energy Application in Agriculture,Shandong Acodemy of Agricultural Sciences,Jinan 250100);Study on Transformation of Wheat and Maize Via Gene-gun Bombardment[J];SHANDONG AGRICULTURAL SCIENCES;1999-02
2 YAO Fang-yin,ZHU Chang-xiang,LIU Li-mei,ZHOU Zhan-yun, LI Guang-xian (Shandong Rice Research Institute, Jining 272077, China);Study on improving embryogenic callus induction and plant regeneration rates from mature embryos in rice[J];SHANDONG AGRICULTURAL SCIENCES;2000-04
3 YANG Ai-fang 1,ZHAO Shi-lan 1,ZHU Li-ping 1,ZHAI Shu-mei 1, ZHANG Ju-ren 1,HUANG Yue 2,ZHAI Xiao-ling 2 (1 Life Science School, Shandong University, Jinan 250100, China;2 Qingdao Agricultural Institute, Qingdao 266100, China);The creation of new sugar beet germplasm tolerant to salt by genetic transformation[J];Shandong Agricultural Sciences;2002-02
4 Zhi Da-ying~1, Xu Chun-hui~1, Xue Zhe-yong~1,Liu Qing-zhong~2, Jiang Hong-min~3, Xia Guang-min~1 (1.Life School of Shandong University, Jinan 250100, China;2.Shandong Institute Pomology,Taian 271000,China; 3.Yantai Academy of Agricultural Sciences, Yantai 265500, China);AFP1 gene transformation to wheat by Agrobacterium tumefaciens[J];Shandong Agricultural Sciences;2004-03
5 LIU Fang~1,Lü Wei-tao~1,CUI De-cai~2,ZHAO Tan-fang~1(1.College of Agronomy,Shandong Agricultural University,Taian 271018,China;2.College of Life Sciences,Shandong Agricultural University,Taian 271018,China);Obtained transgenic wheat induced by anti-PLDγ gene and its primary identification of powdery mildew resistance[J];Shandong Agricultural Sciences;2006-02
6 Gao Junshan et al (Anhui Agricultural University,Hefei 230036);Summary of the Techniques and Methods of Plant Gene Transformafion[J];Journal of Anhui Agricultural Sciences;2003-05
7 Huang Min et al (College of Life Science,Yangtze University, Jingzhou,Hubei 434025);Transformation Research Progress in Maize[J];Journal of Anhui Agricultural Sciences;2004-05
8 LI Feng-ling et al (Rice Research Institute of Sichuan Agricultural University,Wenjiang,Sichuan 611130);Plant Lectin and its Application in the Genetic Engineering of Plant Resistance to Insects[J];Journal of Anhui Agricultural Sciences;2006-03
9 FU Yu-lan et al (Anhui Agricultural University,Hefei,Anhui 230036);Study on the Micropropagation of Dendrobium nobile[J];Journal of Anhui Agricultural Sciences;2006-03
10 HUANG Li et al(Department of Biotechnology and Food Engineering,Hefei University of Technology,Hefei,Anhui 230009);Effect of Metal Ion on the Polysaccharide Synthesis in Protocorm-like Body of Dendrobium huoshanense in Suspension Culture[J];Journal of Anhui Agricultural Sciences;2006-09
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHONG Sheng et al (Institute of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101);Double-gene Expression Vector Construction of Combining Cold-induced Expression Gene CBF3 and Over Expression Gene AtGloS3 and Its Transformation to Arabidopsis thaliana[J];Journal of Anhui Agricultural Sciences;2008-01
2 XUE Li et al(Department of Entomology,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193);Effect of Transgenic Bt plus CpTI Cotton on Carboxylesterase and Acetylcholinesterase of Cotton Aphid Aphis gossypii[J];Journal of Anhui Agricultural Sciences;2011-25
3 LIU Yang1,CHEN Xiao-qiangv1,SUN Ning1,ZHANG Nai-nan1,SUN Jing2,ZHANG Lei1 (1.Plant Cell Engineerning Reasrch Center,Tianjian Agricultural University,Tianjin 300384;2.Domestication and Breeding Rescue Center of Wild Animal in Tianjin,Tianjin 301602);Transgenic Research Progress of Cymbidium hybridum[J];Northern Horticulture;2010-13
4 LIU Jing~1,SHAO Jian-zhu~1,XU Ji-zhong~1,LI Xiang-li~2,GUO Wen-wu~3 (1.College of Horticulture,Agricultural University of Hebei,Baoding 071001,China;2.Biological Department,Jining Teacher's College of Shandong,Jining 272025,China;3.National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China);Study on the transformation of apple with LFY gene mediated by Agrobacterium tumefaciens[J];Journal of Agricultural University of Hebei;2006-03
5 WANG Zhen-yi,WU Li-zhu,WANG Xing-fen,ZHANG Gui-yin,LIU Jian-feng,MA Zhi-ying (North China Key Laboratory for Crop Germplasm Resources of Education Ministry,Key Laboratory of Crop Germplasm Resources of Hebei,Agricultural University of Hebei,Baoding 071001,China);The genetic transformation of embryonic tip via particle bombardment in cotton[J];Journal of Agricultural University of Hebei;2010-03
6 WU Guan-ting, CHEN Jin-qing (Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China);Advances in studies on genetic transformation of tall fescue[J];Pratacultural Science;2006-12
7 ZHANG Fang,WANG Zhou,ZONG Jun-qin,LIU Jian-xiu,SHE Jian-ming(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Establishment of an Agrobacterium mediated genetic transformation system for Eremochloa ophiuroides[J];Acta Prataculturae Sinica;2011-02
8 Wang Cheng(Key Laboratory of Forest Tree Genetic Improvement and Biotechnology,Ministry of Education(Northeast Forestry University),Harbin 150040,P.R.China);Wang Yijun(Heilongjiang Nongken Vocational College);Li Huiyu,Jiang Jing(Key Laboratory of Forest Tree Genetic Improvement and Biotechnology,Ministry of Education(Northeast Forestry University));Effects of Antibiotics on Inhibition of Agrobacterium tumefaciens and Leaf Regeneration of Populus nigra×Populus pyramidalis by Plant Tissue Culture[J];Journal of Northeast Forestry University;2009-09
9 QU Shen-chun,ZHANG Zhen~*,QIAO Yu-shan (College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China);Advance of ZIP gene family related to iron transporter in plant[J];Acta Botanica Boreali-occidentalia Sinica;2004-07
10 LI Xuegui~(1),CHEN Ting~(2),LI Xia~2(1.Department of Biology,Dezhou University,Dezhou Shandong 253023,China;2.Institute of Agrobiological Genetics and Physiology,Jiangsu Academy of AgriculturalSciences,Nanjing Jiangsu 210014,China);Progress in Research on Transforming Technology of Paddy Rice by Agrobacterium[J];Journal of Dezhou University;2005-04
China Proceedings of conference Full-text Database 5 Hits
1 XU Lei-feng~(1,2),FENG Hui-yin~1,LIANG Yuan~1,YUAN Su-xia~1,LIU Chun~1,MING Jun~1 (1 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing,100081,China;2College of life science,Shandong Agricultural University,Taian,271018,China);Advances on the Gene Engineering on Disease-Resistance and Insect-resistance of Lilium[A];[C];2011
2 ;农杆菌介导法将LFY基因导入嘎拉的研究[A];[C];2006
3 HUANG Ting-ting,LAI Lin-lin,WANG Yi,ZHANG Jing-hai,SONG Yong-bo(School of Biological Science and Biopharmacy,Shenyang Pharmaceutical University,Shenyang,110016);Construction of Plant Expression Vector and Transformtion for A.tumefciens of BmK ANEPⅢ Gene[A];[C];2008
4 Wang Kang~1,Dong Jie~2,Zhou He~(1*) (1.Institute of Grassland Science,China Agriculture University,Beijing 100193,China 2.Institute of animal science,CAAS,Beijing 100193,China);Effects of Transgenic Technology on the Molecular Breeding for Turfgrass Plants[A];[C];2010
5 AI Tie-Min, LI Da-Hui ( School of Pharmaceutical Sciences , Peking University , Beijing 100083 , China);PROGRESS ON CHINESE PHARMACEUTICAL BOTANY[A];[C];2003
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