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《Acta Botanica Sinica》 2001-01
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Isolation and Expression Analysis of Two cDNAs Encoding C4H Homologues from Gossypium arboreum

LUO Ping, WANG Guo_Dong, CHEN Xiao_Ya * [WT6BZ](National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200032, China)  
Two cDNAs encoding cinnamate 4_hydroxylase homologues were isolated from Gossypium arboreum L. by PCR_mediated method using a primer based on a conserved region of plant cytochrome P450. The two cotton P450s belong to the subfamily of CYP73A, and were named CYP73A25 and CYP73A26, respectively. Both the cDNAs encode proteins of 505 amino acid residues, showing 91.3% sequence identity with each other. Their sequence identities with C4Hs of other dicotyledon plants may be as high as 90%. RT_PCR analysis indicated that CYP73A26 was clearly expressed in sepals, petals, peels and seeds during development. For CYP73A25, while a significant level of transcripts was detected in sepals, petals and peels, the level in developing seeds was either weak, or undetectable.
【Fund】: 国家自然科学基金!( 3 992 5 0 2 );; 国家高技术发展计划! (Z17_0 4_0 2 )&&
【CateGory Index】: Q943.2
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