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《Acta Botanica Sinica》 2001-01
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Changes in Inhibitory Effect of Vanadate on the Plasma Membrane H~+-ATPase Under Trypsin Treatment

QIU Quan_Sheng *, ZHANG Nan [WT6BZ](College of Life Sciences, Beijing Normal University, Beijing 100875, China)  
The changes in inhibitory effect of vanadate on the plasma membrane H + ATPase were studied with mild trypsin treatment using plasma membrane vesicles purified from soybean ( Glycine max L.)hypocotyles by sucrose gradient centrifugation. Results showed that under mild trypsin treatment the ATPase ATP hydrolysis activity was increased significantly. It was also found that the inhibitory effect of vandate was reduced after proteolysis. In the presence of 2 mmol/L vanadate, the ATP hydrolysis activity of the cleaved ATPase was inhibited by only 53.49%,while that of the un_cleaved ATPase was inhibited by 64.13%. Kinetic studies indicated that both the K m values and the inhibition type of vanadate were not affected by trypsin treatment. Upon proteolysis, K m remained as 0.34 mmol/L,while vanadate was still an uncompetitive inhibitor. Taking together, the structure and activity of the ATPase phosphatase domain were affected by trypsin treatment, implying that this domain might be regulated by the C_terminal end of the plasma membrane H + ATPase.
【Fund】: 国家重点基础研究发展规划项目!(G19990 1170 5 );; 国家自然科学基金项目!( 3 9770 0 77);; 教育部科学技术重点项目!( 9913 3 )资助项
【CateGory Index】: Q945.18
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Xiao-zhi,QIAN Xiao-qing,SHAN Yu-hua,FENG Ke (Coll of Envir Sci and Engin,Yangzhou Univ,Yangzhou 225127,China);Inhibitory effect of vanadate on plasma membrane H~+-ATPase activity of corn roots[J];Journal of Yangzhou University(Agricultural and Life Science Edition);2010-01
【Citations】
Chinese Journal Full-text Database 1 Hits
1 QIU Quan Sheng (Department of Biology, Beijing Normal University, Beijing 100875);STRUCTURE AND FUNCTION OF PLANT PLASMA MEMBRANE H + ATPase[J];CHINESE BULLETIN OF BOTANY;1999-02
【Co-citations】
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4 YANG Ying-li~(1,2),YANG Ning~1,AN Li-zhe~(2,3),ZHANG Li-xin~(3,4)(1 School of Life Science,Northwest Normal University,Lanzhou 730070,China;2 Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of Sciences,Lanzhou 730000,China;3 College of Life Science,Lanzhou University,Lanzhou 730000,China;4 Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China);Research Advances about Plasmalemma H~+-ATPase in Plants[J];Acta Botanica Boreali-Occidentalia Sinica;2006-11
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6 LIU Zhi-hua (Departement of Biology,Hengshui Normal College,Hengshui,Hebei 053000,China);The Structure,Function and Salt-secreted Mechanism of Salt Glands[J];Journal of Hengshui Normal College;2003-01
7 BAO Yong1,2,XU Zheng-yu1,2,ZHU Yi-yong1,2,WANG Yang1,LIU Shi-feng1,DING Chuan-yu1,JIANG Yao1,ZHANG Xiao-man1,XU Guo-hua1(1.College of Resources and Environmental Sciences,2.Life Science Laboratory Center,Nanjing Agricultural University,Nanjing 210095,China);Response of plasma membrane H~+-ATPase activity in roots of oil rape(Brassica napus L.)to phosphate deficiency[J];Journal of Nanjing Agricultural University;2008-02
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【Co-references】
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1 WANG Rong-xin1,LEI Da-wei2,SU Xiao-qing1(School of Basic Medcine,Guiyang Medical College,Guiyang 550004);A primary study on purification of subtilisin-like protease(Pr1) from Pythium guiyangense and its enzymological properties[J];Journal of Anhui Agricultural University;2007-04
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10 KONG Ling-quan1,PU Ying-hui2,MA Shi-kun1,TU Gang1,REN Guo-sheng1 1Department of General Surgery,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China; 2School of Foreign Languages,Chongqing Technology and Business University,Chongqing 400064,China;Better performance of Western blotting:quick vs slow protein transfer,blotting membranes and the visualization methods[J];Journal of Southern Medical University;2008-01
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