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《Acta Botanica Sinica》 2001-10
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PEG-mediated Transformation of Lentinus edodes

SUN Li, XU Wei_Hong, CAI Hua_Qing, HU Yuan_Lei, LIN Zhong_Ping * (National Key Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China)  
Expression vector p301_bG1 contains a gus gene and a bialaphos resistance gene both driven by glyceraldehydes_3_phosphate dehydrogenase (GPD) gene promoter isolated from Lentinus edodes (Berk.) Sing. Using p301_bG1, PEG_mediated transformation of protoplast of L. edodes was studied. Mixed with PEG_purified plasmid DNA, the protoplasts of L. edodes were treated with PEG solution and cultured on CYM regeneration plate containing 40 μg/mL bialaphos. Bialaphos_resistant and GUS_positive transformants were obtained using this transformation system. Although the transformation efficiency was relatively low, the protocols release large expenses on expensive instrument and restriction enzymes, providing a simple and economical method for mushroom breeding at the molecular level.
【CateGory Index】: S646.12
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