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《植物学报(英文版)》 2003-01
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Studies on Membrane Function and Sugar Components of Ultradried Seeds

WANG Xiao-Feng, JING Xin-Ming , LIN Jian, ZHENG Guang-Hua, CAI Zao(1. Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China; 2. Academy of Biology, Beijing Forestry University, Beijing 100083, China)  
The effects of ultradry storage on the activity of ATPase and fluidity of plasma membrane in Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) and elm ( Ulmus pumila L.) seeds were investigated. The results indicated that no significant differences in the activity of ATPase and the micro-viscosity of plasma membrane of ultradried (UD) seeds could be found as compared to the control seeds stored under - 20 ℃ , although there was a little adverse effect on the seeds with extreme dehydration. The results were consistent with higher vigor level of UD seeds. This implied that ultradry seed storage could protect the integrity of the membrane, maintain its physiological function and improve the storability of seeds. The relationship between sugar and desiccation tolerance of UD seeds was analysed using the HPLC. The results showed that the ratio of reducing to nonreducing sugar was lower in UD seeds than that in control. The content of sucrose and stachyose in elm and Chinese cabbage UD seeds was related to their desiccation tolerance, while no stachyose was detected in corn ( Zea mays L.) seeds. This could be one of the reasons for its sensitivity to desiccation.
【Fund】: 国家重大基础研究项目(G2000046806);; 国家基金项目(30170100);; 中国科学院财政部生命科学专项支持~~
【CateGory Index】: Q945.65
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