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《植物学报(英文版)》 2003-01
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Fine Mapping of AST Gene in Arabidopsis

MAO Ai-Jun, WANG Tai , SONG Yan-Ru(Institute of Botany, The Chinese Academy of Sciences , Beijing 100093, China)  
The ast (anthocyanin spotted testa) mutant, which was induced by carbon ion radiation, was a single recessive gene mutant of Arabidopsis thaliana (L.) Heynh. with spotted pigment in seed coats, and involved in the anthocyanin biosynthesis. To clone the AST gene by map-based cloning strategy, a series of molecular markers were designed according to the SNPs (single nucleotide polymophisms) and insertion/deletion polymophisms in the Arabidopsis database. With these molecular markers, the fine-structure mapping of the AST gene was finished, the AST locus was located in BAG clone T13M11. It was suggested that the AST candidate gene was T13M11. 8 in the T13M11. This gene was 1 432 bp long with 6 exons and 5 introns. The putative protein of T13M11. 8 gene was similar to dihydroflavonol 4-reductase (DFR), which was an important enzyme in the anthocyanin biosynthesis pathway.
【Fund】: 中国科学院生物科学与生物技术研究特别支持费课题(财政部专项)(SIZ98-2-14)~~
【CateGory Index】: Q943.2
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