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《植物学报(英文版)》 2003-01
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Ac/Ds Transposition Activity in Transgenic Rice Population and DNA Flanking Sequence of Ds Insertion Sites

ZHU Zheng-Ge, FU Ya-Ping, XIAO Han, HU Guo-Cheng, SI Hua-Min, YU Yong-Hong, SUN Zong-Xiu(1. Key Laboratory for Rice Biology, Ministry of Agriculture, China National Rice Research Institute, Hangzhou 310006, China; 2. College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China)  
The Agrobacterium mediated transgenic rice ( Oryza saliva L.) population with inserts of maize transposon Activator/Dissociation (Ac/Ds) was investigated. DNA sequences flanking the T-DNA were analyzed with inverse PCR. Results showed that 65.4% of the T-DNA was integrated in different locations of rice genome, and some T-DNA flanking sequences were located on certain chromosomes. A number of T-DNA was found to have inserted into protein coding regions. In order to induce transposition of the inserted Ds elements, 354 crosses of Ac x Ds and Ds x Ac were constructed. The excision frequency of Ds element trans-activated by Ac transposase was 22.7% in the F2 populations, and the transposition was confirmed with analyses of DNA sequences flanking the Ds elements. In addition to the transposition due to "cut-paste" mechanism, Ds can replicate itself and integrate into a new locus, and inaccurate excisions were also found. A proportion of DNA segments flanking the Ds elements showed no homologies to sequences published in GenBank, of which two were registered under the accession numbers AF355153 and AF355770. The strategy of using transposon tagging for rice genomics study was discussed.
【Fund】: 国家自然科学基金重点项目(39789020);; 国家重大基础研究发展规划项目(G199901160)~~
【CateGory Index】: S511
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