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《植物学报(英文版)》 2003-01
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Cotton Plants Transformed with the Activated Chimeric Cry1Ac and API-B Genes

GUO Hong-Nian, WU Jia-He, CHEN Xiao-Ying, LUO Xiao-Li, LU Rui, SHI Yue-Jin, QIN Hong-Min, XIAO Juan-Li, TIAN Ying-Chuan(1. Institute of Microbiology, The Chinese Academy of Science, Beijing 100080, China; 2. Institute of Cotton, Shanxi Academy of Agricultural Sciences , Yuncheng 044000, China)  
A chimeric gene, Bt29K, composed of coding sequences of activated Cry1Ac insecticidal protein and an endoplasm reticulum-retarding signal peptide, was synthesized. A plant expression vector containing two expression cassettes for the Bt29K and API-B genes was constructed. These two insect-resistant genes were transferred into two cotton ( Gossypium hirsutum L.) varieties (or lines) via Agrobacteriun-mediated transformation and nine homozygous transgenic cotton lines showing a mortality of 90.0% - 99.7% to cotton hairworm ( Heliothis armigera ) larvae and good agronomic traits were selected through six generations. Molecular biology analysis revealed that one or two copies of the insecticidal protein genes were integrated into the transgenic cotton genome and activated CrylAc and API-B protein expression was at a level of 0.17% and 0. 09% of the total soluble protein in the transgenic cotton leaves, respectively. Comparison of the insect-resistance of the homozygous lines expressing the activated chimeric CrylAc and API-B with that expressing CrylAc only revealed that the insect-resistance of the former is apparently higher than the latter. These results also indicate that the strategy to construct a plant expression vector expressing two different insect-resistant genes reported here is reasonable.
【Fund】: 国家高技术研究和发展计划(863)项目(Z17-01-01;2001AA212071)~~
【CateGory Index】: S562
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