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《植物学报(英文版)》 2003-03
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Growth of Large Single Crystals of Nitrogenase CrFe Protein and MnFe Protein

L Yu_Bing 1, ZHAO Ying 1, ZHAO Jian_Feng 1, DAI Xiao_Hu 2, CANG Huai_Xing 2, WANG Yao_Ping 2 *, HUANG Ju_Fu 1 * (1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China; 2. Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China)  
By using the liquid/liquid diffusion method at a suitable crystallization conditions, large single and dark brown crystals (the sides of the largest crystals were 0.20 mm×0.20 mm×0.07 mm and 0.18 mm×0.18 mm×0.05 mm, respectively) could be obtained from the solutions of nitrogenase CrFe protein and MnFe protein purified from a mutant UW 3 of Azotobacter vinelandii Lipmann grown in Cr_ or Mn_containing but NH 3_free medium. The time of crystal formation, as well as the number, size, shape and quality of crystals obviously depended on the concentrations of PEG, MgCl 2 and NaCl. The liquid/liquid diffusion method seems to benefit CrFe protein and MnFe protein for the growth of large single crystals for X_ray diffraction analysis.
【Fund】: 国家重点基础研究发展规划项目 ( 0 0 1CB10 89_0 6) ;; 国家载人航天工程 ( 92 1_2 );; 国家自然科学基金 ( 3 0 2 70 2 96)~~
【CateGory Index】: Q946
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