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《植物学报(英文版)》 2003-03
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Growth of Large Single Crystals of Nitrogenase CrFe Protein and MnFe Protein

L Yu_Bing 1, ZHAO Ying 1, ZHAO Jian_Feng 1, DAI Xiao_Hu 2, CANG Huai_Xing 2, WANG Yao_Ping 2 *, HUANG Ju_Fu 1 * (1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China; 2. Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China)  
By using the liquid/liquid diffusion method at a suitable crystallization conditions, large single and dark brown crystals (the sides of the largest crystals were 0.20 mm×0.20 mm×0.07 mm and 0.18 mm×0.18 mm×0.05 mm, respectively) could be obtained from the solutions of nitrogenase CrFe protein and MnFe protein purified from a mutant UW 3 of Azotobacter vinelandii Lipmann grown in Cr_ or Mn_containing but NH 3_free medium. The time of crystal formation, as well as the number, size, shape and quality of crystals obviously depended on the concentrations of PEG, MgCl 2 and NaCl. The liquid/liquid diffusion method seems to benefit CrFe protein and MnFe protein for the growth of large single crystals for X_ray diffraction analysis.
【Fund】: 国家重点基础研究发展规划项目 ( 0 0 1CB10 89_0 6) ;; 国家载人航天工程 ( 92 1_2 );; 国家自然科学基金 ( 3 0 2 70 2 96)~~
【CateGory Index】: Q946
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【References】
Chinese Journal Full-text Database 1 Hits
1 SHOU Kai-sheng(Zhejiang Juhua Middle School,Quzhou 324004,China);Cultivation of single crystal for X-ray diffraction test[J];Chemical Engineer;2006-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Shu-jie,LIU Wen-cong,ZHAO Xiao-song~*(College of Resources and Environmental Science,Jilin Agricultural University,Jilin 130118,China);Synthesis and characterization of meso-tetrakis(para-Hydraoxylphenyl) porphyrin and its complexes[J];Journal of Molecular Science;2006-01
2 Li Wei Yuan Guichun (Chemistry Department of HanShan Teachers college, ChaoZhou, 521041;MaoMing Shi Health School, MaoMing,525000);Synthesis of Complex of Hexamethylenetetramine, 1,2-phthalic Acid and Imidazole and Cu(Ⅱ) andCultivation of Single Crystal[J];Guangdong Chemical Industry;2004-07
3 Chen Ziyun,Peng Mengxia (Department of Chemistry, Jiaying University, Meizhou 514015,China);Study on Preparation of Crystals of Ammonium Oxalate Hydrate and Ammonium Hydrogen Oxalate Half-hydrate[J];Guangdong Chemical Industry;2005-04
4 SHI Tong-shun , ZHANG Yuan-fu, YU Lian-xiang, CAO Xi-zhang (Department of Chemistry, Jilin University, Changchun, 130023);Application of Fourier Transform Infrared Photoacoustic Spectroscopy to Porphyrin Studies (Ⅰ)——Fourier Transform Infrared Photoacoustic Spectra ofTransitional Metal Porpbyrin Complexes[J];Chemical Research In Chinese Universities;1992-07
5 CHENG Da-Dian, ZHUANG Yan, WU Zhen-Yu, YANG Shi-Yao LIN Yong-Sheng, ZHAN Meng-Xiong, ZHENG Lan-Sun (Department of Chemistry, Xiamen University, Xiamen, 361005);Synthesis and Characterizations for (η~2-C_(60)) [Pt(PPh_3)_2]_8[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1997-08
6 DONG Zhen Rong, CHENG Da Dian, WU Zhen Yi LI Jian Lin, ZHAN Meng Xiong *, ZHENG Lan Sun(Department of Chemistry, Xiamen University, Xiamen 361005, China);Synthesis and Characterization of Complex η~2-C_(60)[Ru(NO)(PPh_3)] _2[J];Chemical Research In Chinese Universities;2002-06
7 JIA Hui-Ying+1, XU Wei-Qing+{1*}, QI Ming-Hui+2, LIU Guo-Fa+2, LI Xiao-Ling+1, ZHAO Bing+1 ( 1. Key Laboratory of Supramolecular Structure and Materials of Ministry of Education, Jilin University,Changchun 130023, China; 2. College of Chemistry, Jilin University, Changchun 130023, China );Spectral Study of 5,10,15,20-Tetra(p-Chlorophenyl) PorphyrinLanthanide Complexes[J];Chemical Research In Chinese Universities;2004-02
8 ZHANG Heng-Bin;SUN Shu-Qing; LIU Guo-Fa; LI Shu-Jia(Department of Chemistry,Jilin University, Changchun,130023);Electrochemical Study on Acetylacetone-Tetraphenyl Porphyrin Rare Earth Complexes[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1994-12
9 ZHANG Yin-Han;LIU Yun-Ping;NIO Qi-Dao;SONG Zi-Tai(Department of Applied Chemistry,Centre of Structure and Element Analysis,University of Science and Technology of China,IIefei,230026);Synthesis of Copper(Ⅱ)Complex with meso-Tetra(4-hydroxyphenyl)Porphyrin by Solid State Reaction[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-04
10 WANG Xing-Qiao;GAO Shuang;CAO Chang-Sheng;SHI Tong-Shun;YU Lian-Xiang;CAO Xi-Zhang;Department of Chemistry,Jilin University,Changchun,130023);A New Method for Synthesis and Characterization of p-Alkoxyl-phenyl-benzoporphyrin[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-06
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