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《植物学报(英文版)》 2003-09
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Characterization of Endopeptidases in Wheat Leaves During Dark-induced Senescence

RUI Qi, XU Lang-Lai*(College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China)  
The characterization of senescence-associated endopeptidase (EP) isoenzymes in wheat(Triticum aestivum L. cv. Yangmai 158) leaves during dark-induced senescence was performed. It wasfound that there was much higher endoproteolytic activity in dark-induced wheat leaves than in control.Six endopeptidase isoenzymes (EP1-EP6) were identified by natural gradient-polyacrylamide gel electro-phoresis (PAGE) co-polymerized gelatin in the gel, five of which (EP1, EP2, EP4, EP5 and EP6) were onlydetected in senescing leaves. Treatment with 6-benzyl aminopurine (6-BA) delayed the expression ofthese EP isoenzymes and abscisic acid (ABA) accelerated it. The activity of EP3 could be detected at awider range of pH and temperature levels while EP4, EP5 and EP 6 could be only detected at pH 4-5 and 30?5 ℃, EP1 and EP2 at pH 3? and 30?5 ℃. All of the EP isoenzymes showed high thermal stability,especially EP3, EP5 and EP6 which still had activitiy even by incubation at 55 ℃ for 1 h. By using differentclass-specific inhibitors, EP1 and EP2 were characterized as metal-dependent cysteine-proteases, EP4 asa serine-protease.
【Fund】: 高校博士点科研基金资助项目(1999002003)。~~
【CateGory Index】: S512.101
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