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《植物学报(英文版)》 2003-09
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Cytological and Molecular Identification of Alien Chromatin in Giant Spike Wheat Germplasm

DOU Quan-Wen1, 2 , CHEN Pei-Du 1 , XIE Jun-Feng2(1. Key Laboratory of Crop Cytogenetics of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;2. Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining 810008, China)  
Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding,genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random ampli-fied polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried botha pair of wheat-rye (Triticum aestivum-Secale cereal ) 1BL/1RS translocation chromosomes and a pair ofAgropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three otherscarried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome.Further identification revealed that the identical Ai chromosome in these germplasm lines substituted thechromosome 2D of common wheat (T. aestivum L.), designated as 2Ai. The genetic implication and furtherutilization of 2Ai in wheat improvement were also discussed.
【Fund】: 国家自然科学基金(39893350)。~~
【CateGory Index】: S512.101
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