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Study on Judgement of Maturity Degree by Free Proline Content in Flue-cured Tobacco Leaves

ZHANG Guan-li1,NIE Rong-bang2 (1 Shaoyang Tobacco Company,Shaoyang,Hunan,422000,China;2 College of Agronomy,HNAU,Changsha,Hunan,410128,China)  
In order to explore the physiological and biochemical indexes which could be used to judge the maturity degree of flue-cured tobacco leaves in the field,the free proline in tobacco leaves was extracted with sulfosalicylic acid and mensurated with spectrophotometer.The results showed that the free proline content in tobacco leaves showed "V" shape from unripe to overripe.The time when the lowest proline content in the leaves appeared was the optimum harvest stage.The results suggested that the free proline content in leaves can be used as a index to determine the optimum harvest date,and this method was reliable,simple and direct.
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