Genetic Diversity Analysis of 16 Genotypes of Houttuynia cordata Thunb by SRAP
WANG Kun1,ZHONG Jun2,3,,ZHANG Dan-dan2,QIU Ping3,ZENG Wei-jun3(1 College of Bioscience and Technology,HNAU,Changsha,Hunan 410128,China;2 College of Agronomy,HNAU,Changsha,Hunan 410128,China;3 Hunan Zhengqing Pharmaceutical Co.LTD,Huaihua,Hunan 418000,China)
The SRAP amplified and reaction systems were researched and SRAP-PCR map of 16 genotypes of Houttuynia cordata Thunb were constructed and a UPGMA dendrogram was obtained by applying DPS software.The results were as follows:(1) The best reaction system was 40 ng DNA,0.1 mmol/L dNTPs,2.0 mmol/L Mg2+,37.5 ng/μL primer and 2 U Taq polymerase in 25 μL system.(2) 118 pairs of primers were selected from 360 pairs and 7 582 bands were amplified,of which 6 590 were polymorphic,and the polymorphic percentage of bands was 86.92%.(3) In amplified products of Me9-GA18,specific deletion band of genotype ZY06-028 was at 250 bp,and which of ZY06-016 and ZY06-028 were at 550 bp.In amplified products of Me8-Em10,specific deletion band of ZY06-42 and ZY06-01 was at 300 bp and 450 bp respectively.These results can be used to identify genotypes of Houttuynia cordata Thunb.(4) At the genetic distance of 0.31,16 genotypes of Houttuynia cordata Thunb could be divided into 3 groups,in which ZY06-024 and ZY06-01 were significantly different from other types,so they were clustered into one group respectively,and the rest genotypes were clustered into one group.