LYMPH NODE TRANSPLANTATION IN RATS: CHANGES IN CELLULAR ARCHITECTURE AND POSSIBLE INFLUENCE ON ITS HISTOLOGICAL AND FUNCTIONAL RESTORATION
Liu Ning. fei, et al. Shanghai Institute of Plastic Surgery, Shanghai Second Medical University, Shanghai. 200011
The cellular, architectural and functional changes of transplanted mesenteric lymph nodes in the rats were studied.After the lymph nodes were transplanted with interrupting both of afferent and efferent lymphatics, the nodes gradually depleted the celluar content. One month after operation the recirculating lymphoeytes in the transplanted node were 3.5% of that in the host nodes, while the number of cells of the transplanted node was only 28%. The number of macrophages also decreased obviously. In paracortex, the cells of high endothelial venule (HEV) became flattened and HEV disappeared gradually. In the cortex, the germinal centers and follicles also diminished considerably in number and disappeared completely in 3 month. It was difficult to distinguish the normal compartments in the operated nodes 3 months after transplantation. No regenerated afferent lymphatics were identified in the transplanted nodes. Lacking of antigen and stimulated lymphocytes brought histological and functional involution of the transplanted lymph nodes.The effect of Corynebacterium Parvum (C.P) and interleukin 2 (IL-2) on the regeneration of the transplanted lymph node was observed. The injection of C. P (3mg/week) subcutaneously in the skin of antieipated lymph drainage region showed noinfluence on the lymph node and lymphatics. The grafted node underwent a progress ofinvolution. The injection of IL-2 (500u twice a week ) brought about more recirculating lymphocytes in the node, although the immunological function remained absent in the node. This effect may help to the maintenance of the intergrity of transplanted lymph node.