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Cysteine Determination Using Cobalt (Ⅱ) Ionprobe

Zhang Guizhu, Wang Yuemei, Zhao Peng, He Xiwen and Shi Huiming (Department of Chemistry, Nankai University, Tianjin 300071, China) Lu Jixin (Department of Pharmacy, Tianjin Medical University, Tianjin 300203, China) [WT4”HZ〗  
Based on the reaction of cysteine and cobalt (Ⅱ) in NH 4Cl NH 4OH solution, a new spectrophotometric method for the determination of cysteine was developed. The molar absorptivity is 7.98×10 3 L·mol -1 ·cm -1 at 284 nm and the results met the Beer′s law in the range of 0~2.0×10 -4 mol·L -1 . The method was applied successfully in cysteine assay in urine.
【CateGory Index】: Q517,
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