Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of New Drugs》 2013-09
Add to Favorite Get Latest Update

The in vitro anti-virus effects and dose-effect relationship of epigoitrin and fructopyrano-(1→4)-glucopyranose based on "deletion/increment" strategy

ZHANG Shi-jing1,LIU Ming-hua1,LI Han-bing2,JIANG Li1,LUO Ying1,SUN Qin1(1 School of Pharmacy,Luzhou Medical College,Luzhou 646000,China; 2 Henan University of TCM,Zhengzhou 450008,China)  
Objective: To investigate the in vitro anti-virus effects and dose-effect relationship of epigoitrin and fructopyrano-(1→4)-glucopyranose(FG) from the traditional Chinese medicine Isatidis radix.Methods: Inhibitory effect of the target components on influenza virus neuraminidase(NA) was determined by fluorescent enzyme immunoassay.The 50% inhibitory concentration(IC50) was used as an indicator to research the relationship between epigoitrin and FG by equivalent curve analysis.The effective concentrations of epigoitrin and FG for in vitro anti-virus activity were determined.In the negative samples,the concentration range of target composition was gradually increased to obtain the key efficacy("incremental").Results: The equivalent point of epigoitrin and FG samples was located below the equivalent line,and the IC50,mix value was less than IC50,add.For anti-influenza A virus activity of the incremental samples of epigoitrin and FG,the effective concentrations were as follows: the lowest,optimum,and highest concentrations of epigoitrin were 82.5,225.0 and 289.7 μg·mL-1;those of FG were 138.2,450.0 and 618.9 μg·mL-1.Conclusion: The equivalent curve analysis shows that epigoitrin and FG exhibit cooperation.We have,for the first time,revealed the effective concentration range for in vitro anti-virus activity of epigoitrin and FG from Isatidis radix.
【Fund】: 国家自然科学基金(30873381)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 CHENG Yan,LI Xiang,CHEN Jian-wei,HE Li-wei,WANG Ying-ying (School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,210046,China);A Study on Antiviral Effects of the Active Parts from Radix Isatidis[J];Journal of Nanjing University of Traditional Chinese Medicine;2011-02
2 CAO Hong-peng 1, TAO Pei-zhen 1, DU Guan-hua 2 (1. Institute of Medicinal Biotechnology; 2. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China);ESTABLISHMENT AND APPLICATION OF HIGH THROUGHPUT SCREENING MODEL FOR INFLUENZA VIRUS NEURAMINIDASE INHIBITORS IN VITRO[J];Acta Pharmaceutica Sinica;2002-12
3 LI Han-bing1, 2, YAN Dan1, WANG Jia-bo1, WANG Jing-yan3, BEI Zhu-chun3, WEI Li1, 2, XIAO Xiao-he1(1. Institute of Chinese Medicine, 302 Hospital of People’s Liberation Army, Beijing 100039, China; 2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China; 3. State Key laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China);Biological evaluation of Radix Isatidis based on neuraminidase activity assay[J];Acta Pharmaceutica Sinica;2009-02
4 XU Li-Hua~(1*),HUANG Fang~(2),CHENG Ting~(3),WU Jie~(4) ()~(1)School of Pharmacy,Soochow University,Suzhou 215007;()~(2)School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 210038;()~(3)School of Life Science,Nanjing University,Nanjing 210093;()~(4)Suzhou Siyuan Medicine Technology Co.,Ltd.,SuZhou 215011,China;Antivirus Constituents of Radix of Isatis indigotica[J];Chinese Journal of Natural Medicines;2005-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XIONG Qing-ping et al(School of Chinese Materia Medica,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510405);Purification Technology of Epigoitrin from ISATIDIS RADIX by Macroporous Resin[J];Journal of Anhui Agricultural Sciences;2011-30
2 KUANG Zhi-zhou1,ZHENG Li-shu1,DUAN Zhao-jun1,QI Zheng-yu1,QU Xiao-wang1,LIU Wen-pei1,ZHANG Wan-ju1,LI Dan-di1,GAO Han-chun2,HOU Yun-de1 (1.National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China;2.Beijing Jindike Bio-tech Institute,Beijing 100176,China);Screening of Peptides as Broad-spectrum Neuraminidase Inhibitors against Influenza Viruses[J];Chinese Journal of Virology;2007-03
3 AN Yi-qiang1,2,JIA Xiao-bin1,2,CHEN Yan1,GAO Cheng-lin1,SHAO Zhen-zhong1 (1Jiangsu Provincial Key Laboratory of New Drug Delivery System & Applied Technology of Chinese Materia Medica, Nanjing 210028,China;2School of Pharmacy,Jiangsu University,Zhenjiang 212013,China);Determination on content of epigoitrin in Banlangen granules from different pharmaceutical factories by RP-HPLC[J];China Journal of Traditional Chinese Medicine and Pharmacy;2009-04
4 AN Yi-qiang1,2, JIA Xiao-bin1,2, CHEN Yan-hua3, GAO Cheng-lin1 (1Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Key Laboratory of Scientific Preparation for Clinical Effective Prescription of State Administration of Traditional Chinese Medicine, Nanjing 210028,China; 2School of Pharmacy, Jiangsu University, Zhenjiang 212013,China; 3School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046,China);Content determination of 2,4(~1H,~3H)-quinazolinedione in Radix Isatidis from different regions by HPLC[J];China Journal of Traditional Chinese Medicine and Pharmacy;2010-02
5 WEI Lan-ping,CHEN Jian-xin,HE Zi-hua,LU Chun-ni,LI Jin-feng,HUANG Xian-hui,FANG Bing-hu(College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China);Effect of Hypericin on Influenza Virus H9N2 in Vitro[J];Progress in Veterinary Medicine;2010-12
6 LUO You-hua,LI Cheng-fu,YANG Hui,HUANG Kai-fei,HUANG Yi-qi.Xiamen Medical Institute,Xiamen 361003,China;Study on optimizing of the alcohol precipitation refining technology for Yanshuning compound water extract[J];Fujian Medical Journal;2010-05
7 XIN Rui-hua1,LUO Yong-jiang1,ZHENG Ji-fang1,WANG Gui-bo1,LUO Chao-ying1,XIE Jia-sheng1,HU Zhen-ying1,LI Jin-yu1,LI Wei 2(1.Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;2.Lanzhou Center for Disease Control and Prevention,Lanzhou 730030,China);Effects of Genhuang Dispersible Tablets on Part of the Physiological and Biochemical Function of Broilers[J];China Animal Husbandry & Veterinary Medicine;2010-11
8 WANG Lin-qing1,2,CUI Bao-an2,3,ZHANG Hong-ying2,3(1.Life Science Department,Zhengzhou Normal University,Zhengzhou 450044,China;2.Key Laboratory of Henan Province Animal Food Safety,Zhengzhou 450002,China;3.College of Animal Husbandry and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China);Antiviral effect of Flos Lonicerae japonica and Flos lonicerae flavonoids ingredients to PRV in vitro[J];China Animal Husbandry & Veterinary Medicine;2011-03
9 SUN Xiao-bing,SHENG Jia-rong,WANG Ding-pei(Department of Chemistry,Guangxi Teachers Education University,Nanning 530001,China);Research Progress of Chemical Constituents and Pharmacological Activities for Baphicacanthus cusia (Nees) Bremek[J];Journal of Guangxi Teachers Education University(Natural Science Edition);2008-04
10 MENG Han-fu,CHEN Xiao-jun,LIANG Shan-dan,HUANG Yuan,LIAO Jiu-ming,PANG Ji-mei,LIANG Xia(Guangxi Boke Pharmaceutical Co.,Ltd.,Guangxi Nanning 530007,China);HPLC for Determining the Content of Epigoitrin in Compound Banlangen Oral Solution[J];Guangzhou Chemical Industry;2012-13
China Proceedings of conference Full-text Database 7 Hits
1 JIA Xiaobin1,2, SHI Feng1,2,3, CHEN Yan1,2, AN Yiqiang1, LU Yang4, WANG Hengbin4 (1.Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China; 2. The Key Unit of Chinese Medicine DDS of SATCM, Nanjing 210028, China; 3. Jiangsu University, Zhenjiang 212013, Jiangsu, China; 4. Suzhou LeiYunShang ,Suzhou 215006, Jiangsu, China);A New Thread and Practice for Investigating the Antiviral Effect Substance of Traditional Chinese Medicine Against Respirovirus[A];[C];2010
2 Xiao Xiao-he,Yan Dan,Yuan Hai-long Wang Jia-bo (China Military Institute of Chinese Materia Medica,302 Military Hospital of China, Beijing 100039);The novel patterns of efficient constituent recognition and quality control for Chinese medicines based on component knock-out & knock-in[A];[C];2009
3 XIN Rui-hua~1,LUO Yong-jiang~1,ZHENG JI-fang~1,WANG Gui-bo~1,LUO Chao-ying~1,XIE Jia-sheng~1,HU Zhen-ying~1,LI Jin-yu~1,LI Wei~2 (1.Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,Lanzhou 730050,China; 2.Lanzhou Center for Disease Control and Prevention,Lanzhou 730030,China);Effects of Genhuang Dispersible Tablets on part of the physiological and biochemical function of broilers[A];[C];2010
4 Guo Zhi-ting~1,WEI Xu-bin~2,LIANG Jian-ping~1,LUO Xiao-qin~3 (1.Key Laboratory of New Animal Medicine Project,Lanzhou Institute of Animal Husbandry & Veterinary Pharamaceutics Science,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;3.Lanzhou Institute of Animal Health Inspection,Lanzhou 730050,China);Study of hypericin soluble powder on CDV in vitro[A];[C];2010
5 Yang Fan,Liu Ailin,Du Guanhua (Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China);Study of standardization of influenza neuraminidase inhibition assay[A];[C];2011
6 JIA Xiaobin~(27,2),SHI Feng~(1,2,3),CHEN Yan~(1,2),AN Yiqiang~1,LU Yang~4,WANG Hengbin~4 (1.Key Laboratory of New Drug Delivery System of Chinese Meteria Medica,Jiangsu Provincial Academy of Chinese Medicine, Nanjing,210028,China; 2.The Key Unit of Chinese Medicine DDS of SATCM,Nanjing,210028,China; 3.Jiangsu University,Zhenjiang,212013,China; 4.Suzhou LeiYunShang,Suzhou,China,215006);A new thread and practice for investigating the antiviral effect substance of Traditional Chinese Medicine against respirovirus[A];[C];2010
7 LU Fang-guo,ZHANG Bo,LI Ling,TAN Lu,ZHOU Fang-liang,ZHOU Sai-nan,DING Huang,GE Zi-yu Traditional Chinese Medicine University of Hunan,Changsha 410208,China;Neuraminidase activity of Maxing Shigan Decoction on mice which were infected by influenza virus[A];[C];2013
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Sheng 2, CHEN Wan Sheng 1, QIAO Chuan Zhuo 1, ZHENG Shui Qing 1, ZENG Ming 1,ZHANG Han Ming 1, SONG Zhao Jun 1 (1. Department of Pharmacognosy, College of Pharmacy, Second Military Medical University, Shanghai 200433, China; 2. Department;Antiviral action of Radix Isatidis and Folium Isatidis from different germplasm against influenza A virus[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;2000-03
2 WANG Ying-ying, LI Xiang, CHEN Jian-wei, CHENG Yan, WANG Sheng(Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China);Study on Chemical Constituents from Water Extra of Radix Isatidis[J];Research and Practice on Chinese Medicines;2009-02
3 LIU Hai li 1, WU Li jun 1, LI Hua 2, WANG Jun 3 (1. School of Traditional Chinese Materia Medicas, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Huabang Medicinal and Pharmaceutical Co.,Ltd., Shenyang 110021, China; 3.The Red C;Study on the chemical constituents of Isatis indigotica Fort.[J];Journal of Shenyang Pharmaceutical University;2002-02
4 Li ying LiuHong YeBei (*TongHua City People Hospital, TongHua JiLin 134000, P.R China.);The effects of Radix isatidis on influenza virus FM1 in Kunming mice[J];China Healthcare Innovation;2008-08
5 LIU Yun hai 1, QIN Guo wei 2, FANG Jian guo 1, WU Xiao yun 2(1. Department of Pharmacy, Tongji Hospital Affiliated to the Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; 2. Shanghai Institute of;Screening of Chemical Constituents with Antiendotoxin Activity from Radix Isatidis[J];Herald of Medicine;2002-02
6 LIU Yun hai, LIN Jian guo, LEI Ting(Department of Pharmacy, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China);Effect of active part from Radix Isatidis on content of TNF_α and NO induced by LPS in mice serum[J];Chinese Traditional and Herbal Drugs;2003-02
7 XU Li-Hua~(1*),HUANG Fang~(2),CHENG Ting~(3),WU Jie~(4) ()~(1)School of Pharmacy,Soochow University,Suzhou 215007;()~(2)School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 210038;()~(3)School of Life Science,Nanjing University,Nanjing 210093;()~(4)Suzhou Siyuan Medicine Technology Co.,Ltd.,SuZhou 215011,China;Antivirus Constituents of Radix of Isatis indigotica[J];Chinese Journal of Natural Medicines;2005-06
8 HE Li-wei,LI Xiang,CHEN Jian-wei,ZHANG Jun-feng,SUN Dong-dong,WANG Ying-ying(Nanjing University of Traditional Chinese Medicine,Nanjing 210046,China);Screening of the Antiviral Active Parts from Isatis indigotica[J];China Pharmacy;2008-33
9 LIU Yun-Hai, FANG Jian-Guo,XIE Wei Tongji Hospital,Tongji Medical college,Huazhong University of Science, Technology-Pharmacy, Wuhan 430030, China;Studies on the Antiendotoxic mechanism of Radix Isatidis[J];Journal of China Pharmaceutical University;2003-05
10 Liu Yunhai(Tongji Hospital, Tongji Medical University,Wuhan 430030);Studies on the Antiendotoxic Action of Radix isatidis[J];JOURNAL OF CHINA PHARMACEUTICAL UNIVERSITY;1995-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved