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《Chinese Journal of New Drugs》 2013-09
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The in vitro anti-virus effects and dose-effect relationship of epigoitrin and fructopyrano-(1→4)-glucopyranose based on "deletion/increment" strategy

ZHANG Shi-jing1,LIU Ming-hua1,LI Han-bing2,JIANG Li1,LUO Ying1,SUN Qin1(1 School of Pharmacy,Luzhou Medical College,Luzhou 646000,China; 2 Henan University of TCM,Zhengzhou 450008,China)  
Objective: To investigate the in vitro anti-virus effects and dose-effect relationship of epigoitrin and fructopyrano-(1→4)-glucopyranose(FG) from the traditional Chinese medicine Isatidis radix.Methods: Inhibitory effect of the target components on influenza virus neuraminidase(NA) was determined by fluorescent enzyme immunoassay.The 50% inhibitory concentration(IC50) was used as an indicator to research the relationship between epigoitrin and FG by equivalent curve analysis.The effective concentrations of epigoitrin and FG for in vitro anti-virus activity were determined.In the negative samples,the concentration range of target composition was gradually increased to obtain the key efficacy("incremental").Results: The equivalent point of epigoitrin and FG samples was located below the equivalent line,and the IC50,mix value was less than IC50,add.For anti-influenza A virus activity of the incremental samples of epigoitrin and FG,the effective concentrations were as follows: the lowest,optimum,and highest concentrations of epigoitrin were 82.5,225.0 and 289.7 μg·mL-1;those of FG were 138.2,450.0 and 618.9 μg·mL-1.Conclusion: The equivalent curve analysis shows that epigoitrin and FG exhibit cooperation.We have,for the first time,revealed the effective concentration range for in vitro anti-virus activity of epigoitrin and FG from Isatidis radix.
【Fund】： 国家自然科学基金(30873381)
【CateGory Index】： R285.5
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