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《Chinese Journal of New Drugs》 2013-21
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Determination of antioxidant activity in licorice vitro metabolites by DPPH spiking coupled with HPLC-Q-TOF MS/MS

LUO Li-ping;LI Yan-jing;ZHANG Hui;LI Ping;CHEN Jun;State Key Laboratory of Natural Medicines,China Pharmaceutical University;  
Objective: To investigate the antioxidant activity of liver microsomal metabolites from six flavonoids in licorice by 1,1-diphenyl-2-picrylhydrazyl(DPPH) spiking coupled with HPLC-Q-TOF MS / MS.Methods: The six flavonoids were incubated with rat liver microsomes and the resultant metabolites were identified by HPLC-Q-TOF MS / MS.The antioxidant activity of the metabolites was calculated by comparing the peak area changes before and after incubation with DPPH.Results: The peak area of the metabolites significantly decreased or even disappeared after incubation with DPPH.Besides the metabolites Lico A-M1 and Lico A-M2 of licochalcone A,more than 90% of other metabolites of flavonoids were oxidized.Conclusion: Liver microsomal metabolites of echinatin,licochalcone B,licochalcone A,5-(1,1-dimethylally)-3,4,4'-trihydroxy-2-methoxychalcone,glycycourmarin and glyurallin B still have significant antioxidant activities.
【Fund】: 国家自然科学基金(81073006);; 教育部新世纪优秀人才计划(NECT-09-0769)
【CateGory Index】: R285
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