Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of New Drugs》 2013-21
Add to Favorite Get Latest Update

Determination of antioxidant activity in licorice vitro metabolites by DPPH spiking coupled with HPLC-Q-TOF MS/MS

LUO Li-ping;LI Yan-jing;ZHANG Hui;LI Ping;CHEN Jun;State Key Laboratory of Natural Medicines,China Pharmaceutical University;  
Objective: To investigate the antioxidant activity of liver microsomal metabolites from six flavonoids in licorice by 1,1-diphenyl-2-picrylhydrazyl(DPPH) spiking coupled with HPLC-Q-TOF MS / MS.Methods: The six flavonoids were incubated with rat liver microsomes and the resultant metabolites were identified by HPLC-Q-TOF MS / MS.The antioxidant activity of the metabolites was calculated by comparing the peak area changes before and after incubation with DPPH.Results: The peak area of the metabolites significantly decreased or even disappeared after incubation with DPPH.Besides the metabolites Lico A-M1 and Lico A-M2 of licochalcone A,more than 90% of other metabolites of flavonoids were oxidized.Conclusion: Liver microsomal metabolites of echinatin,licochalcone B,licochalcone A,5-(1,1-dimethylally)-3,4,4'-trihydroxy-2-methoxychalcone,glycycourmarin and glyurallin B still have significant antioxidant activities.
【Fund】: 国家自然科学基金(81073006);; 教育部新世纪优秀人才计划(NECT-09-0769)
【CateGory Index】: R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 YANG Hua1,2,HAN Kun1,WAN Xiao-chun1,FANG Cong-bing 1,3 (1.Key Laboratory of Tea & Medicinal Plants and Product Safety,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036; 2.School of Science,Anhui Agricultural University,Hefei 230036;3.School of Horticulture,Anhui Agricultural University,Hefei 230036);Free radical scavenging capacities of isoflavonoid glycosides isolated from Pueraria Radix[J];Journal of Anhui Agricultural University;2011-02
2 WANG Hong1,2,CHEN Jun-Hui1,ZHAO Heng-Qiang1,WANG Lei-Lei1,2,ZHANG Dao-Lai1,WANG Xiao-Ru1,3,YANG Dong-fang21(QingDao Key Lab on Analytical Technology Development and Standardization of Chinese Medicines,First Institute Oceanography of State Oceanic Administration,Qingdao 266061)2(College of Aqua-life Science and Technology,Shanghai Ocean University,Shanghai 201303)3(Department of Chemistry and the Key Laboratory of Analytical Science of the Ministry of education,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005);On-line Screening and Identification of Free Radical Scavenging Compounds in Water Extract of Tea by High Performance Liquid Chromatography-Mass Spectrometry-2,2-Diphenyl-1-Picrylhydrazyl[J];Chinese Journal of Analytical Chemistry;2009-06
3 PEI Shi-chun1,XU Jiu-liang2,UM Byunghun2,SHANG Ya-fang2(1.College of Food and Biological Engineering,Qiqihar University,Qiqihar 161006,China;2.Natural Product Research Center,KIST Gangneung Institute,Gangneung 210-340,Korea);Screening of Antioxidant Components from Ledum palustre L. Leaves by Online HPLC-ABTS+Method[J];Food Science;2012-19
4 HAN Yao-dan1,WANG Bin2,WANG Zheng-yu1,ZHANG Ya-tao1, YANG Lin-yun1,ZHOU Jin-pei1,ZHANG Hui-bin1(1 Center of Drug Discovery,China Pharmaceutical University,Nanjing 210009,China; 2 R&D Center,Jiangsu Yabang Pharmaceutical Group,Changzhou 213200,China);Recent research progress in pharmacological effects of glycyrrhizic acid[J];Chinese Journal of New Drugs;2012-21
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XU Li,SHI Le,XU Li(Nanjing University of Traditional Chinese Medicine,Nanjing 210046,China);Research methods on material basis of traditional chinese medicine compound[J];Anhui Medical and Pharmaceutical Journal;2010-01
2 ZHENG Shi-rui,LI Jun,SONG Jue(School of Pharmacg,Anhui Medical University,Provincial Laboratory of The Activity Research of Natural Medicines in Anhui,Three-level Laboratory of Scientific Research of Traditional Chinese Medicine of State Administration of Traditional Chinese Medicine of People's Republic of China "Pharmacology of Traditional Chinese Medicine",State and Provincial Key Laboratony,Hefei,Anhui 230032,China);The new ideas about the pharmacokinetics study of traditional Chinese compound medicine[J];Anhui Medical and Pharmaceutical Journal;2010-11
3 Zhan Hongsheng Zhejiang College of TCM,Hangzhou(310009);Experimental Method of Medicinal Serum and Its Application Prospects in the Study and Making of New Chinese Drugs[J];JOURNAL OF ZHEJIANG COLLEGE OF TCM;2000-02
4 MIAO Chun sheng 1, ZHAO Li yan 2, ZHAO Xue mei 1, ZHANG Xiu yun 1, LI Cai 1* (1.Dept.of Pathology, Institute of Frontier Medical Sciences, Jilin University, Changchun 130021, China;2.Dept. of Laboratory,The Second Hispital, Jilin University);Study on Inhibitory Effect of Tangshen′an on Glycation in Vitro[J];Journal of Jilin University of (Medicine Edition);2003-06
5 ZHONG Xun-long1, GUO Jiao1, 2, DING Jin-long1, HE Wei1( 1Key Unit of Modulating Liver to Treat Hyperlipemia SATCM, Three-level laboratory of Lipid Metabolism SATCM, Institute of Chinese Medical Science of Guangdong Pharmaceutical University, Guangzhou, 510006, China; 2Guangzhou University of Chinese Medicine, Guangzhou, 510006, China );Serum pharmacochemistry study on Fufang Zhenzhu Tiaozhi Capsule[J];China Journal of Traditional Chinese Medicine and Pharmacy;2011-06
6 YANG Si-Min1,CHEN Rui-Zhan1,DONG Hang1,LI Yuan1,LI Shi-Zhe1, LI Xin-Long1,SONG Feng-Rui2,LIU Zhi-Qiang2 1(College of Chemistry,Changchun Normal University,Changchun 130032,China) 2(Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China);Rapid Screening and Structures Characterization of Antioxidants from Methanol Extract of Scutellariae Radix by High Performance Liquid Chromatography-Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2012-06
7 DING Jin-long, GUO Jiao, PIAO Sheng-hua (Institute of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China);Serum Pharmacochemistry and Its Prospective Application on Research and Development of Functional Food[J];Modern Food Science and Technology;2008-06
8 XU Ming-zhe;Department of Agricultural Resources and Environment,College of Agriculture,Yanbian University;;Study of the Antioxidant Activity of Periostracum Cicadae Extracts Based on DPPH Free Radical Scavenging Capability[J];Journal of Anhui Agricultural Sciences;2013-24
9 Li QingpingSan-ai Medicine Ltd., Fujian Province;Pondering over the Research into Material Base of Traditional Chinese Medical Formulae[J];Journal of Henan College of Traditional Chinese Medicine;2004-01
10 ZHOU Li-qin,WANG Han-min,CHEN Lin-na,et al(The Hubei Hospital of Traditional Chinese Medicine,Wuhan 430061);Traditional Chinese Medicine Bacteriostatic Effect on Drug-resistive Isolates: Observations under the Electron Microscope[J];Journal of Hubei College of Traditional Chinese Medicine;2006-01
China Proceedings of conference Full-text Database 1 Hits
1 WANG Yu, YANG Yong-qing, Chen Han-ping, CUI Jian-mei, MA Shu-lan. (Shanghai Institute Research of Acupuncture and Meridians, Shanghai, 200030; Shanghai University of Traditional Chinese Medicine, Shanghai, 200030; Shanghai Medical Collage of Fudan University, 200032, China. *.the Communication Author);The Advance of Acupuncture-serum Research[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MA Xue-mei (School of Biotechnology and Food Engineering,Anyang Institute of Technology,Anyang,Henan 455000);Research Progress on Ledum plasture as A Resource Plant[J];Journal of Anhui Agricultural Sciences;2010-06
2 YANG Hua1,2,HAN Kun1,WAN Xiao-chun1,FANG Cong-bing 1,3 (1.Key Laboratory of Tea & Medicinal Plants and Product Safety,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036; 2.School of Science,Anhui Agricultural University,Hefei 230036;3.School of Horticulture,Anhui Agricultural University,Hefei 230036);Free radical scavenging capacities of isoflavonoid glycosides isolated from Pueraria Radix[J];Journal of Anhui Agricultural University;2011-02
3 Yu Jinghua,Zhao Dan,Fu Yujie,Kong Yu,Yuan Shusheng,Zu Yuangang(Key Laboratory of Forest Plant Ecology(Northeast Forestry University),Ministry of Education,Harbin 150040,P.R.China);Content Determination of Four Components in Ledum palustre Leaves by RP-HPLC[J];Journal of Northeast Forestry University;2011-03
4 GUO Rui1,ZHANG Yun1,LIU Ya-jing1,WANG Jun-ru2 (1 College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China;2 College of Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China);Isoflavone Content and Antioxidant Activity of Ethanol Extract from Flowers of Pueraria lobata[J];Acta Botanica Boreali-Occidentalia Sinica;2009-06
5 GENG Xue-fei2,ZHENG Yong-jie1,ZHAO Ming2,UM Byung-hun3 (1.College of Chemistry and Chemical Engineering,Qiqihar University,Qiqihar 161006,China;(2.Natural Product Research Center,KIST Gangneung Institute,Gangwon 210-340,Korea);Screening of antioxidant components by HPLC-ABTS system in Ledum palustre L[J];Chemical Engineer;2011-10
6 ZHU Qing Lei,HE Ai Xia ,LU Xin Ran ( Applied Pharmacology Laboratory, Weifang Medical College,Weifang 261042,Shandong) ( Department of Anesthesiology,The Affiliated Hospital of Weifang Medical College,Weifang 261031,Shandong);Effects of Puerarin on the Scavenge of Oxygen Free Radicals and the Antagonism Against Oxidative Injury[J];Pharmaceutical Journal of Chinese People's Liberation Army;2001-01
7 ZHANG Yan,LIU Tao,LUO Su-qin,et al.(Inner Mongolia Medical College,Hohhot 010059 China);THE RESEARCH PROGRESS ON CHEMICAL COMPOSITIONS AND PHARMACOLOGICAL EFFECTIONS OF LEDUM PALUSTRE[J];Acta Academiae Medicinae Neimongol;2010-S3
8 LIU Rong1,CAO Shu-wen1,YU Yan-ying2,DENG Ze-yuan1,(1. State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China; 2. Department of Chemistry,Nanchang University,Nanchang 330031,China);Free Radical Scavenging Activities of Genistein and Its Lactosides[J];Food Science;2008-09
9 LIN Lian-zhu,ZHAO Mou-ming (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China);Effect of Reaction Time on DPPH and ABTS+ Radical Scavenging Assays for Antioxidant Capacity Evaluation[J];Food Science;2010-05
10 DAI Jun, WANG Hong xin, CHEN Shang wei, TANG Jian (Wuxi University of Light Industry, Wuxi 214036, China);Study on the Analytical Methods of Catechins in Tea and Green Tea Polyphenol Samples by High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;2001-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved