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《Chinese Pediatrics of Integrated Traditional and Western Medicine》 2017-04
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Effect of different dose of astragalus injection on the Caspase-3 activity of mice with viral myocarditis

ZHANG Yunjuan;GOU Hongping;NIU Shaomin;DONG Xiangyu;Department of Pediatric Cardiology,Lanzhou University Second Hospital;  
Objective To observe the effect of astragalus injection on cell apoptosis in mice with viral myocarditis and on the Caspase-3 activity.Methods A total of 60 male Balb/c mice(4 to 6 weeks old)were randomly divided into 5 groups:normal control group(A),model control group(B),low-dose astragalus group(C),middle-dose astragalus group(D)and high-dose astragalus group(E),12 mice in each group.After the mice model of acute viral myocarditis was made,astragalus injection was injected into the abdominal cavity of the mice in group C,D and E[0.1,0.2,0.4mL/(g·d)],while normal saline was injected in group A and B[0.1mL/(g·d)],once a day for continuously 14 days.On day 3,7,10,and 14,heart and serum samples were taken for pathological testing and the detection of Caspase-3 activity,CK-MB and cTnI.Results(1)Myocardial pathological score,and the value of CK-MB and cTnI of mice in group B were significantly higher than those of group A,the difference being statistical(P0.05).(2)The Caspase-3 activity,CK-MB,cTnI value and myocardial pathological score in group C,D and E were significantly lower than those in group B,with significant difference(P0.05).(3)There was no statistical difference on myocardial pathological score,CK-MB or Caspase-3 activity among group C,D and E(P0.05).Conclusion (1)Cell apoptosis is involved in the myocardial damage of mice with viral myocarditis,and Caspase-3 plays an important role in it.(2)Small-dose astragalus injection can reduce the apoptosis of myocardial cells in mice with viral myocarditis by down-regulating the Caspase-3 activity of myocardial cells,and thus alleviate the damage to myocardial cells.
【Fund】: 兰州大学第二医院院内中医药科研项目(YJzy2013-20)
【CateGory Index】: R285.5
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