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《Zhejiang Journal of Preventive Medicine》 2015-01
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A study on the mechanism of biofilm formation of Pseudomonas aeruginosa and its relationship with drug resistance

SHEN Jian;FENG Chen;LIN Yun-zhao;ZHENG Wen-hua;GU Hua;JIANG Jin-qin;FENG Lei;Zhejiang Medical College;  
Objective To compare antibiotic resistance of Pseudomonas aeruginosa in biofilms and in planktonic state,so as to provide practical guidance and theoretical support for clinical treatment of drug. Methods Pseudomonas aeruginosa were divided into control culture group( group A),medium group of biological membrane after culture( group B) and the biofilm culturing silica film adhesion group( group C). Three groups were observed by silver staining,extracellular sugar staining and scanning of electron microscope. These groups were cultured with different antibiotic medium,and the situation of bacterial resistance was observed by the method of k-B. Results There was no significant difference in drug resistance and minimum inhibitory concentration( MIC) between group A and B( P 0. 01). Compared with group A,group C showed that their drug resistance on meropenem,cefepime,ceftazidime,ceftriaxone,amoxicillin and levofloxacin were much higher( P 0. 01). The minimal biofilm eradication concentration( MBEC) of group C was about 100 times than that of group A and group B. Conclusion The assay of establishment of pseudomonas aeruginosa biofilm and the methods observed by silver stainingin vitro is convenient and feasible. At the same time,pseudomonas aeruginosa in biofilm exhibits far more resistance to antimicrobial than its planctonic counterparts does.
【Fund】: 浙江省医药卫生科学研究基金(2006A021);; 浙江医学高等专科学校研究基金(2005XZ09)
【CateGory Index】: R378.991
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