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Analysis of Pinus massoniana Pollen Polysaccharide Composition with HPLC

Lu Nannan1,Shi Lihua2,Liu Hui1,Liu Zhihe2,Geng Yue1(1.College of Life Science of Shandong Normal University,Jinan 250014,China;2.New Era Health Industry International Development Co.,Ltd,Yantai 264000,China)  
Objective To compare the monosaccharide composition of polysaccharide with 40%,60%,80% concentration ethanol precipitation,which were isolated from Pinus massoniana pollen.Method The polysaccharides was hydrolyzed by sulfuric acid,then derivatized by 1-pheny-3-methyl-5-pyrazolone(PMP),and determined by HPLC method at 250nm wavelength.We obstained separation of the monosaccharide with clear peaks using KH2PO4(pH 5.6)-acetonitrile gradient elution,they are all separated in baseline.Result Our experiment showed that glucose,galactose,arabinose and glucuronic acid mainly constituted the monosaccharide composition of polysaccharide,and we got the molar proportion of monosaccharide content by calculating peak areas.
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