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Determination of Cholorgenic aid in Galium verum L.from different areas by HPLC

Lu Wen-hong,Yan Fan,Yv Xiao-min,et al  
object To establish and compare the determination and stability of Cholorgenic aid in Galium verum L.from 10 different arears,and lay the foundation fou medicinal materials and preparation.Method:HPLC was performed on ODS C 18 colunm.the mobile phase was MeOH-H 2O-H 3PO 4 with a gradient program and detected at 340nm.Results:The regression equations of Cholorgenic aid was C=0.73×10 -6A+0.00425 r=0.9994,and the average recovery rates was95.8%.Conclusions:the determination of Cholorgenic aid in Galium verum L.from 10 different arrears are difference,and the Cholorgenic aid should not be the main target composition of quality standard of Galium verum L.Author's address:Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China
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