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《Chinese Journal of Medical Imaging Technology》 2010-01
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Ultrasound-targeted microbubble mediated liposome small interference RNA transfection to retinal pigment epithelium cells

LI Hong-li 1,DU Lian-fang 1,ZHENG Xiao-zhi 1,WANG Hui-ping 2,GU Qing 3,CHEN Xia-fang 2(1.Department of Ultrasound,2.Experimental Research Center,3.Department of Ophthalmology,Shanghai First People's Hospital,Shanghai Jiao Tong University,Shanghai 200080,China)  
Objective To investigate the transfection efficiency of combination of ultrasound(US)microbubbles(MBs)mediated liposome small interference RNA(siRNA)to human and rat retinal pigment epithelium(RPE)cells.Methods Human and rat RPE cells and siRNA were incubated in 24-well plates(2×10 5/well and 1×10 5/well,respectively).The cells were devided into 5 groups:siRNA+US,siRNA+MBs+US,siRNA+L(liposome),siRNA+L+US,siRNA+L+US+MBs.After 12 h,transfection efficiency was observed with fluorescence microscopy and flow cytometry.Results US or ultrasound targeted microbubbles destruction without liposome-mediated could not promote siRNA transfection efficiency to human and rat RPE cells.The transfection efficiency of human and rat RPE cells significantly decreased in the siRNA+L+US+MBs group,but increased in siRNA+L+US group.Conclusion Ultrasonic irradiation can promote lipid-mediated siRNA transfected human RPE cells.
【Fund】: 国家自然科学基金(30772369)
【CateGory Index】: R774.1
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【References】
Chinese Journal Full-text Database 1 Hits
1 LI Hong-li1,DU Lian-fang1,WANG Hui-ping2,GU Qing3,CHEN Xia-fang2(1.Department of Ultrasound,2.Experimental Research Center,3.Department of Ophthalmology,Shanghai Jiao Tong University Affiliated First People's Hospital,Shanghai 200080,China);Ultrasound and perfluoropropane-albumin microphere promote the infection efficiency of adeno-associated virus to human retinal pigment epithelium cells in vitro[J];Chinese Journal of Medical Imaging Technology;2010-05
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Wu Ying,Du Lianfang,Gu Qing,et al Department of Medical Ultrasonics,Shanghai Jiaotong University Affiliated First People's Hospital,Shanghai 200080 China;Ultrasound and SonoVue Enhanced pEGFP-N1 Transfection to Retinal Pigment Epithelium:In Vitro Experimental Study[J];Chinese Journal of Ultrasound in Medicine;2007-05
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3 TIAN Li1,LI Ji-chang2,ZHAO Guo-qiang3,YUAN Jian-jun4,CHEN Kui-sheng5,CAI Qing-chun1(1.Department of Ultrasound,the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China;2.Department of Gastroenterology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;3.Laboratoty of Microbiology & Immunology,Zhengzhou University,Zhengzhou 450052,China;4.Department of Ultrasound,Henan Provincial People's Hospital,Zhengzhou 450003,China;5.Henan Key Laboratory of Tumor Pathology,Zhengzhou 450052,China));Effect of microbubble taking TIMP-1 siRNA to liver fibrosis of rats under ultrasound irradiation[J];Chinese Journal of Medical Imaging Technology;2007-07
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 WANG Qing-song, FANG Xiao-ling (Department of pharmacy,Fudan University, Shanghai 200032,China);Prospects for Nonviral Carriers in Gene Therapy Against Cancer[J];Pharmaceutical Biotechnology;2005-02
2 LUO Liang 1, LI Li-jun 1* , CHENG Yong-qing 2, ZHANG Xue-jiao 2, WANG Yi-li 3 (1.Department of Emergency, the Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China; 2. Applied Chemistry Department, the Science College of Northwest Industry University, Xi'an 710072, China; 3. Pathology Department, Medical School of Xi'an Jiaotong University, Xi'an 710061, China);Ability of ultrasound contrast agent microbubles to carry DNA[J];Chinese Journal of Medical Imaging Technology;2005-04
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 LUO Jie,WANG Zhi-gang*(Institute of Ultrasound Imaging,Chongqing University of Medical Sciences,Chongqing 400010,China);Progress of ultrasonic molecular imaging of hepatocellular carcinoma[J];Chinese Journal of Interventional Imaging and Therapy;2009-06
2 CHEN Zhi-yi,XIE Ming-xing,WANG Xin-fang,LV Qing(Department of Ultrasonography,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Province Key Laboratory of Molecular Imaging,Wuhan 430022,China);Enhancement of EGFP gene transfection to rat myocardium:ultrasound-targeted microbubble destruction combined with PEI[J];Chinese Journal of Medical Imaging Technology;2009-01
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Tang Qing,Xu Huixiong,Lv Mingde,et al Department of Medical Ultrasonics, First Affiliated Hosipital,Sun Yat-Sen University,Guangzhou 510080 China;Ultrasound Exposure Mediated Transfection of pEGFP to HUVEC:the Enhancement Role of an Ultrasound Contrast Agent (SonoVue)[J];Chinese Journal of Ultrasound In Medicine;2005-05
2 RAN Hai-tao~1, REN Hong~2, WANG Zhi-gang~(1*), ZHENG Yuan-yi~1, ZHANG Qun-xia~1, XU Chuan-shan~1 (1.Institute of Ultrasonic Image, Chongqing University of Medical Sciences, Chongqing 400010, China; 2.Institute of Viral Hepatitis of Chongqing Medical University, Chongqing 400010, China);Experimental study of enhanced gene delivery to cultured tumor cells in vitro by ultrasound and contrast agent[J];Chinese Journal of Medical Imaging Technology;2005-08
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