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《The Journal of Traditional Chinese Orthopedics and Traumatology》 2015-01
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Application of joint fluid metabolomics to diagnosis and COLD-HEAT SYMPTOM COMPLEX typing in patients with rheumatoid arthritis

HU Gangfeng;XIAO Luwei;TONG Peijian;The First People's Hospital of Xiaoshan district in Hangzhou;Zhejiang Chinese Medical University;Zhejiang Provincial hospital of TCM;  
Objective: To explore the value of joint fluid metabolomics in diagnosis and COLD-HEAT SYMPTOM COMPLEX typing of rheumatoid arthritis( RA). Methods: The knee joint fluid of 12 patients with RA( COLD- DAMPNESS BLOCK COLLATERALS( 寒湿阻络) in 6 patients and DAMPNESS-HEAT BLOCK COLLATERALS( 湿热阻络) in 6 patients) and 7 healthy volunteers were collected respectively and gas chromatography-mass spectrometry( GC-MS) was performed on the samples. The principal component analysis( PCA) was performed on GC- MS data of selected main chromatographic peak. Results: Twenty-three main chromatographic peaks were selected as common peaks for describing the chemical composition characteristics of joint fluid. The general characteristics of GC-MS total ion chromatogram of healthy volunteers was basically the same as that of RA patients. Twenty-three chromatographic peaks for describing the chemical composition characteristics of joint fluid were integrated into four principal components by using PCA. The PCA were performed on joint fluid samples of all the subjects. The scores of joint fluid samples of RA patients were characterized by concentrated distribution in the third and fourth principal component score plots,while those of healthy volunteers were characterized by relative dispersion. The PCA were performed only on joint fluid samples of RA patients,and the results showed that the scores of joint fluid samples were characterized by relative dispersion distribution in both patients with DAMPNESS- HEAT BLOCK COLLATERALS and patients with COLD- DAMPNESS BLOCK COLLATERALS in the third and fourth principal component score plots. Conclusion: The metabolome data of knee joint fluid can be used to accurately distinguish RA patients from healthy volunteers and distinguish DAMPNESS-HEAT BLOCK COLLATERALS from COLD-DAMPNESS BLOCK COLLATERALS,therefore it provides a new way for diagnosis and TCM symptom complex quantitative classification of RA.
【Fund】: 浙江省中医药管理局项目(2007GA002)
【CateGory Index】: R259
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