Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《The Journal of Traditional Chinese Orthopedics and Traumatology》 2015-11
Add to Favorite Get Latest Update

Impact of Taohong Siwu Tang(桃红四物汤) on Delta-like 4 expression in the autologous skin graft model of rabbits

LIN Nianci;SUN Shaoqiu;LI Yiliang;XU Wuji;CHEN Xilong;ZHOU Zhaohui;Traditional Chinese Medicine Hospital of Xinhui district in Jiangmen City;The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine;  
Objective: To observe the impact of Taohong Siwu Tang( 桃红四物汤,THSWT) on Delta-like 4 expression in the autologous skin graft model of rabbits. Methods: Seventy-five New Zealand rabbits which ranged in age from 10 to 12 months were randomly divided into sham-operated group,model group,low-dose group,middle-dose group and high-dose group,15 cases in each group. The autologous skin transplantation surgery were performed on rabbits in model group,low-dose group,middle-dose group and high-dose group,while sham operation were performed on rabbits in sham-operated group. After the modeling operation,the rabbits in sham-operated group and model group were intragastric administrated with distilled water; while the others in low-dose group,middle-dose group and high-dose group were intragastric administrated with the prepared THSWT in dosages of 2. 5,5 and 10 g / kg at a time,twice a day. Five rabbits were randomly selected from each group and the blood samples were obtained from the heart at 2,5 and 8 days after the beginning of the drug intervention respectively. The rabbits were executed and their anastomotic skin graft tissues were fetched out. The serum content of Delta-like 4 protein were measured by using enzyme-linked immunosorbent assay( ELISA). The acquired skin tissues were divided into 2 parts,and one of them were maded into paraffin sections and the blood vessel density were measured after HE staining,while the other portion were applied to measure the Delta-like 4 mRNA level by using reverse transcription-polymerase chain reaction( RT-PCR). Results: There was no statistical difference in serum content of Delta-like 4 protein between the 5 groups at 2 days after the beginning of the drug intervention( 30. 58 + /-3. 56,32. 31 + /- 2. 66,33. 53 + /- 1. 75,36. 00 + /- 1. 49,35. 63 + /- 1. 87 ng / ml,F = 2. 669,P = 0. 062). There was statistical difference in serum content of Delta-like 4 protein between the 5 groups at 5 and 8 days after the beginning of the drug intervention( 30. 56 + /- 1. 35,33. 52 + /- 2. 50,37. 96 + /- 1. 83,48. 74 + /- 2. 34,49. 05 + /- 2. 86 ng / ml,F = 46. 239,P = 0. 000; 29. 26 + /- 2. 20,32. 98 + /- 1. 43,35. 24 + /- 1. 97,38. 02 + /- 1. 72,36. 06 + /- 2. 50 ng / ml,F = 8. 394,P = 0. 000). There was no statistical difference in serum content of Delta-like 4 protein between model group and sham-operated group and between model group and low-dose group( 5d: P = 0. 112,P =0. 269; 8d: P = 0. 340,P = 0. 182). The serum content of Delta-like 4 protein were higher in middle-dose group and high-dose group compared to model group( 5d: P = 0. 000,P = 0. 000; 8d: P = 0. 020,P = 0. 006). There was no statistical difference in serum content of Deltalike 4 protein between middle-dose group and high-dose group( P = 0. 862,P = 0. 245). The serum content of Delta-like 4 protein were higher in middle-dose group and high-dose group compared to low-dose group at 5 days after the beginning of the drug intervention( P =0. 000,P = 0. 000). There was no statistical difference in serum content of Delta-like 4 protein between middle-dose group and low-dose group and between high-dose group and low-dose group at 8 days after the beginning of the drug intervention( P = 0. 105,P = 0. 623). There was statistical difference in mRNA level of Delta-like 4 in skin graft between the 5 groups at 2,5 and 8 days after the beginning of the drug intervention( 0. 26 + /- 0. 03,0. 29 + /- 0. 04,0. 34 + /- 0. 06,0. 42 + /- 0. 03,0. 39 + /- 0. 04,F = 7. 072,P = 0. 001; 0. 25 + /- 0. 03,0. 34 + /- 0. 04,0. 39 + /- 0. 03,0. 48 + /- 0. 06,0. 45 + /- 0. 06,F = 12. 028,P = 0. 001; 0. 25 + /- 0. 06,0. 30 + /- 0. 04,0. 35 + /- 0. 03,0. 44 + /- 0. 03,0. 43 + /- 0. 04,F = 10. 813,P = 0. 000). There was no statistical difference in Delta-like 4 mRNA level between model group and sham-operated group and between model group and low-dose group( 2 d: P = 0. 452,P = 0. 162; 5 d: P = 0. 320,P = 0. 158; 8 d:P = 0. 171,P = 0. 219). The Delta-like 4 mRNA level were lower in model group compared to middle-dose group and high-dose group( 2 d:P = 0. 000,P = 0. 001; 5 d: P = 0. 001,P = 0. 009; 8 d: P = 0. 001,P = 0. 002). There was no statistical difference in Delta-like 4 mRNA level between middle-dose group and high-dose group( P = 0. 426,P = 0. 330,P = 0. 725). There was no statistical difference in Delta-like 4mRNA level between middle-dose group and low-dose group and between high-dose group and low-dose group at 2 days after the beginning of the drug intervention( P = 0. 111,P = 0. 167). The Delta-like 4 mRNA level was higher in middle-dose group and high-dose group compared to low-dose group at 5 and 8 days after the beginning of the drug intervention( 5 d: P = 0. 024,P = 0. 025; 8 d: P = 0. 013,P =0. 028). There was statistical difference in the number of blood vessels in skin graft under the optical microscope( × 400) between the 5groups at 2 and 5 days after the beginning of the drug intervention( 5. 37 + /- 0. 36,2. 55 + /- 0. 41,2. 83 + /- 0. 24,3. 31 + /- 0. 26,3. 53 + /- 0. 67,F = 4. 084,P = 0. 014; 5. 62 + /- 0. 56,4. 25 + /- 0. 68,4. 80 + /- 0. 74,5. 88 + /- 0. 42,5. 92 + /- 0. 48,F = 4. 600,P =0. 009). The number of blood vessels was less in model group compared to sham-operated group( P = 0. 001,P = 0. 011),and there was no statistical difference in the number of blood vessels between model group and low-dose group( P = 0. 527,P = 0. 268) and between middledose group and high-dose group( P = 0. 620,P = 0. 939). At 2 days after the beginning of the drug intervention,there was no statistical difference in the number of blood vessels between middle-dose group and model group( P = 0. 102) and between high-dose group and model group( P = 0. 131) and between middle-dose group and low-dose group( P = 0. 297) and between high-dose group and low-dose group( P =0. 352). At 5 days after the beginning of the drug intervention,the number of blood vessels was more in middle-dose group and high-dose group compared to model group( P = 0. 003,P = 0. 003),while no statistical difference was found between middle-dose group and low-dose group and between high-dose group and low-dose group( P = 0. 038,P = 0. 033). At 8 days after the beginning of the drug intervention,there was no statistical difference in the number of blood vessels in skin graft under the optical microscope( × 400) between the 5 groups( 6. 18 +/- 0. 33,5. 86 +/- 0. 66,6. 03 +/- 0. 40,6. 27 +/- 0. 53,6. 27 +/- 0. 59,F = 0. 334,P = 0. 852). Conclusion: THSWT can up-regulate Delta-like 4 expression and promote angiogenesis in autologous skin graft model of rabbits. However,we cann't clarify the relationship between the role of THSWT in up-regulating Delta-like 4 expression and its dose and action time,which need to be further studied.
【Fund】: 国家自然科学基金项目(81373658)
【CateGory Index】: R285.5;R-332
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 DONG Da-li 1,ZHOU Zhao-hui 2,CHEN Xi-long 2,PENG Li-tian 2,SUN Da-wu 2(1.2010 Master's Candidate,Hunan University of Chinese Medicine,Changsha 410007,Hunan,China;2.The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,Hunan,China);Mechanism of revascularization of random flap in rats treated with Taohong Siwu decoction[J];湖南中医杂志;2013-05
2 SUN Shao-qiu1,LI Yi-liang2,CHEN Meng-jiao1,CHEN Xi-long1,ZHANG De-sheng1(1.The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005,Hunan,China;2.2010 Master's Candidate at Hunan University of Traditional Chinese Medicine,Changsha 410007,Hunan,China);Effect of Taohong Siwu pills on expression of vascular endothelial growth factor at different time points after skin autograft transplantation in rabbits[J];湖南中医杂志;2013-03
3 LIU Zhu-qing,YIN Deng-ke,HAN Lan,LI Bai-kun,PENG Dai-yin(School of Pharmacy,Anhui University of Traditional Chinese Medicine,Anhui Key Laboratory of Modern Chinese Medicine,Heifei 230031,China);Protective effect of medicated serum prepared with Taohong Siwu Tang on hydrogen peroxide-induced human umbilical vein endothelial cells[J];中国中药杂志;2013-03
4 LI Yi,PENG Dai-yin(Anhui College of Traditional Chinese Medicine,Anhui Key Laboratory of Colleges and Universities for Modern Materia Medica,Hefei,Anhui 230038,China);Progress in pharmacological research of Taohong Siwu Decoction[J];安徽医药;2011-05
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 WANG Jian-jun;WU Jing-dong;The First Clinical College of Liaoning University of Traditional Chinese Medicine;Xi'an Haitang Vocational College Academic Affairs Office;The Office of Academic Affairs,Liaoning University of Traditional Chinese Medicine;;Clinical observation on the treatment of 60 cases of solar lentigo with Huoxuemei granule[J];中国美容医学;2016-06
2 CHEN Jianmei;GUO Jiao;Institute of Chinese Medical Science,Guangdong Pharmaceutical University,Guangdong Metabolic Diseases Research Center of integrated Chinese and Western Medicine;;Progress on the serum pharmacology of Chinese medicine[J];广东药学院学报;2016-03
3 LIU Xiao-yun;ZHAO Xiu-mei;ZHANG Gui-xian;Tianjin University of Traditional Chinese Medicine;;Fuzheng Jiedu Quyu on Postoperative Colorectal Cancer Chemotherapy Synergistic Effect[J];中国中西医结合外科杂志;2016-02
4 GU Zhong-min;LIN Shao-bin;LIU Cai-jun;QI Xin-xin;Department of Intensive Care Unit,Fuzhou Second Hospital Affiliated to Xiamen University;;Effect of Taohong Qinlian Decoction on HMGB1 in Septic Rat Cardiac Muscle[J];中国中西医结合杂志;2016-04
5 HUANG Jing-jing;SUN Shao-qiu;LI Yi-liang;The Second Affiliated Hospital of Hu'nan University of Chinese Medicine;;Research Progress of Angiogenesis after Skin Grafting, Notch Signaling Pathway and the Effects of TCM[J];中医药导报;2016-07
6 YU Yijiang;HONG Bing;CHEN Lijuan;YIN Xuechao;Department of Endocrinology,Huai'an Hospital of Traditional Chinese Medicine;;Taohongsiwu decoction combined with Trimetazidine on patients with type 2 diabetic cardiomyopathy of yin deficiency and blood stasis syndrome and oxidative stress[J];吉林中医药;2016-02
7 HE Keqiang;ZHANG Shijun;TONG Mingou;The Second Traditional Chinese Medicine Hospital of Sichuan Province;;Clinical Observation of Using Modified Taohong Siwu Decoction Combined with Fire Needle to Treat Varicose Veins of Lower Extremity[J];四川中医;2016-02
8 LIANG Rui-feng;LI Kai-yan;WANG Xiao-li;WANG Jun;Henan Provincial Academy of Traditional Chinese Medicine;;Protective Effect of Medicated Serum Prepared with Jiangya Baolan Tablets on Human Umbilical Vein Endothelial Cells Injured by Hydrogen Peroxide[J];中国实验方剂学杂志;2016-03
9 FANG Xiao-lin;ZHOU Bin;Department of TCM Chiropractic;Liwan Hospital of Guangzhou Medical College;Department of Orthopedic Surgery,Liwan Hospital of Guangzhou Medical College;;The Effect of Ozone Combined with Taohongsiwu Decoction on Treatment of Senile Patients with Knee Osteoarthritis[J];医学综述;2016-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 CHANG Jin1,LIU Guo-lin2,LIU Shuo3,SHI Pei-kai4,DONG Fu-sheng4(1.Cosmetology Center of Clinical Medical,Hebei University of Engineering,Handan 056002,Hebei,China;2.Department of Oral and Maxillofacial Surgery,Cangzhou Central Hospital;3.Department of Dental,First Hospital of Handan;4.Stomatology College,Hebei Medical University);VEGF in skin expansion expression and its significance[J];中国美容医学;2012-13
2 YIN Deng-ke,YANG Ye,YIN Juan-juan,HAN Lan,XU Fan,PENG Dai-yin(School of Pharmacy,Anhui University of Traditional Chinese Medicine;Key Laboratory of Research & Development of Chinese Medicine in Anhui Province Hefei 230031,China);Effect of Taohong Siwu Decoction on VEGF and endostatin release from platelet by proteinase-activated receptors agonist[J];中成药;2012-09
3 Zhou Zhaohui1,Liu Jia2,sun Dawu1(1.Hu Nan university of traditional Chinese medicine the second affiliated hospital,hu Nan Chang Sha 410005; 2.Hu Nan university of traditional Chinese medicine 2004 grade seven years system following the even read, Hu Nan Chang Sha 410208);Pink four things soup at survival of rats flap influence[J];中医药导报;2011-07
4 Liao Xiaobo,Zhu Tao,Lin Jiaxin Guangxi College of Chinese Medicine,Nanning,Guangxi,China 530001;Summize of External Chinese Drug Repairing Infected Wound[J];中医学报;2010-05
5 JIN Xiang,SHI Le,WANG Shan-shan,et al (Nanjing University of Chinese Medicine,Nanjing,210046);Influence of taohongsiwutang different extracts on microcirculation of model rats with blood deficiency-blood stasis[J];安徽医药;2010-04
6 GONG Shao-bo1,LI Xiang-qi2,SONG Ai-li3,CHI Li-ping1,SONG Yong-gang1 (1.Shandong College of Traditional Chinese Medicine,Laiyang Shandong 265200;2.Clinical School,Taishan Medical College,Taian Shandong 271000;3.Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan Shandong 250011);Impacts of Yukui Ointment on Expressions of VEGF and bFGF in Ulcerative Granuloma Tissue on Rat Body Surface[J];世界中西医结合杂志;2010-01
7 PU Li-Juan,and LIU Yi(Department of Pharmacology,Liaoning Medical College,Jinzhou 121000,China);Protective Effect of Acetaminophen on Human Umbilical Vein Endothelial Cells Induced by Hydrogen Peroxide[J];中国动脉硬化杂志;2009-09
8 LI Ning~1,XIE Xing-wen~2,Song Min~1 (1.Department of Traditional Chinese Medical,Gansu College of Traditional Chinese Medicine, Lanzhou 730000,China;2.Department of Articular Orthopedics,Traditional Chinese Medicine Hospital of Gansu Province,Lanzhou 730050,China);Effects of Taohongsiwu decoction and Simiao decoction on the expressions of interleukin-1β and interleukin-6 in rat knee osteoarthritis[J];兰州大学学报(医学版);2009-02
9 LIU Dong,TANG Qin-li,LI Yu-bao,PENG Dai-yin(Anhui College of Traditional Chinese Medicine,Anhui Hefei 230038,China);An Experimental Study on Effects of Taohong Siwu Decoction for Primary Dysmenorrhea[J];安徽中医学院学报;2009-02
10 QI Zhen-xi1,KANG Jing-dong2,LI Shu-qiang2(1. Technology Industry Department,FUTCM,Fuzhou,Fujian 350003,China;2. Orthopaedic and Traumatology Department,FUTCM,Fuzhou,Fujian 350108,China);Effect of Taohong Siwu Decoction on Transcription of Transforming Growth Factor-β_1 mRNA of Hormonal Avascular Necrosis of Femoral Head in Rats[J];福建中医学院学报;2009-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved