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Effect of Early Intervention with Fangxiaoyin Granules on Eosinophils and Airway Inflammation in Asthmatic Mice

BIN Boping 1,LI Mingzhong 1,YIN Li 2,HUANG Xiaoqi 1,LIANG Liying 1,ZHANG Xiliu 1,YI Min 1 (1.The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning530023;2.Guangxi University of Chinese Medicine)  
Objective To investigate the effect and mechanism of early intervention with Fangxiaoyin Granules (Granules for preventing asthma) on airway inflammation in asthmatic mice.Methods Fifty healthy BALB/c mice were randomized into 5 groups,i.e.normal control group,asthma model group,Fangxiaoyin Granules small-dose group,Fangxiaoyin Granules large-dose group,and budesonide (BUD) group,with 10in each.The asthmatic models were established with ovalbumin (OVA) sensitization for inspiration.The Fangxiaoyin Granules small-dose group and large-dose group were given small-dose (500g/L crude medicine) and large-dose (2500g/L crude medicine) of Fangxiaoyin Granules respectively through gastric feeding from the first day of sensitization,once a day,0.4ml each time,26days in all.The normal control group and asthma model group were given normal saline in the equal amount through gastric feeding.The BUD group was given BUD solution (0.5g/L) for nasal dripping since the 23rd day under the ethyl ether inhalation anesthesia,0.01ml/day,for 3days.Forty-eight hours after the last inspiration,the mice were put to execution.The contents of serum interleukin-4 (IL-4),interleukin-5 (IL-5),interfer-on-γ (IFN-γ),and interleukin-12 (IL-12) were measured,and the eosinophils (EOS) infiltration in the lung and marrow EOS% were observed with photoelectric microscope.Results In the asthma model group,the HE dyeing of the lung tissues showed that there were massive inflammatory infiltration,mainly EOS and neutrophils;the marrow EOS%,serum IL-4/IFN-γratio,and IL-5/IL-12ratio were significantly increased in comparing with those of the normal control group (P0.01).In the Fangxiaoyin Granules large-dose group and BUD group,the marrow EOS% was significantly decreased,the airway and perivascular inflammatory infiltration was significantly inhibited,and serum IL-4/IFN-γratio and IL-5/IL-12ratio were significantly decreased,the difference between the two groups was not statistically significant (P0.05).Conclusion Fangxiaoyin Granules could effectively down-regulate serum IL-4/IFN-γratio and IL-5/IL-12ratio,decreasing the marrow EOS%,and inhibiting the airway inflammation mainly with EOS infiltration.
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