DNA Barcoding in Genus Piper

HAO Chaoyun1,2,3,WU Huasong1,2,3,FAN Rui1,2,3,YANG Jianfeng1,2,3 WU Gang1,2,3,MA Tengfei4,QIN Xiaowei1,2,3 1 Spice and Beverage Research Institute,CATAS,Wanning,Hainan 571533,China 2 Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops,Ministry of Agriculture,Wanning,Hainan 571533,China 3 Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulatioin for Tropical Spice and Beverage Crops,Wanning,Hainan 571533,China 4 School of Life Sciences,Yunnan University,Kunming,Yunnan 650091,China  

In order to screen right DNA regions in Piper,four candidate DNA barcodes with three(rbcL,psbJpetA,matk) from chloroplast genome and one(ITS) from the nuclear genome,were evaluated in view of 35 selftesting accessions and 282 ones from NCBI.Capability of the four candidate DNA barcodes was evaluated by effectiveness,intra-and inter-specific divergence and barcoding gap analysis,and the identification efficiency was also assessed using Neighbour-Joining method.The results showed that ITS and matK candidate barcodes had clear barcoding gap.At the same time,matK had high species identification reliability,and ITS had high significant divergence at species level.The other two candidate barcodes had no clear barcoding gap.matK and ITS might be the potential DNA barcoding for the identification of Piper plants,thus making the establishment of new identification methods for this genus possible.

【Key Words】： Piper DNA barcoding ITS rbcL psbJ-petA matK
【Fund】： 国家自然科学基金项目(No.31201263);; 农业部南亚热作专项(No.12RZZY-13)
【CateGory Index】： S573.9

Download(CAJ format) Download(PDF format)

CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.