Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Liriodendrin protects SH-SY5Y cells from dopamine-induced cytotoxicity

Da-Long Zhao1,2, Da-Wei Shen3, Yu-Tao Chi3, Fang Liu3, Li-Bo Zou2 and Hai-Bo Zhu1* 1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine affiliated Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China; 2.Shenyang Pharmaceutial University, Shenyang 110016, China; 3.Yanbian University, Yanji 133000, China  
Aim To investigate the effect of liriodendrin, an extract from Fraxinus sielboldiana blume belonging to the Oleaceae family, on dopamine-induced cytotoxicity in human neuroblastoma SH-SY5Y cells. Methods Cell viability was processed when treated with 50μmol·L–1 of dopamine for 24 h by MTT assay. Early apoptosis, late apoptosis/necrosis were analyzed by flow cytometry using Annexin V-FITC and propidium iodide (PI) double-staining, respectively. Generation of reactive oxygen species (ROS) was assessed by DCFH-DA, an oxidation-sensitive fluorescent probe. To evaluate mitochondrion membrane potential (ΔΨm) using flow cytometry with the fluorescent dye Rhodamine 123. The transcriptional level of P53 was studied using RT- PCR. Results The dopamine-induced loss of cell viability was significantly attenuated by liriodendrin treatment at the concentra- tion of 10–8, 10–7, 10–6, 10–5 and 10–4 mol·L–1. The protective effects of liriodendrin (10–7, 10–6 and 10–5 mol·L–1) on dopamine-in- duced cytotoxicity may be ascribed to its anti-oxidative properties by reducing ROS level and anti-apoptotic effect via protection of ΔΨm. In addition, the effect of liriodendrin may involve the P53 pathway in apoptosis. Conclusion Liriodendrin may provide a useful therapeutic strategy for the treatment of neurodegenerative diseases such as Parkinson’s disease (PD)
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved