Analysis on Factors Influencing Pollen Germination and Tube Growth of Solanum tuberosum L.in vitro
YANG Jia-suo et al (Zhuanglang Agriculture and Animal Husbandry Administrative Bureau,Zhuanglang, Gansu 744600)
[Objective] The research aimed to study the influence factors of potato pollen germination and pollen tube growth in vitro.[Method] Potato pollen was gathered and cultivating liquid was confected,the method of liquid media to cultivate pollen was taken in order to research the effects of sucrose,PEG4000,inorganic ion Ca2+、K+、Mg2+、Na+、Zn2+ and plant growth regulating substances with different mass concentrations on pollen germination and pollen tube growth.[Result] The results showed that the most suitable liquid culture medium for potato pollen germination and tube growth in vitro was 10% sucrose+10% PEG4000+0.02% Ca(NO3)2+0.02% H3BO3+0.500 mmol/L K+,and with 0.500 mmol/L Mg2+ and 0.100 mmol/L Na+ could speed pollen germination,especially tube growth.Plant growth regulating substances 6-BA would stimulate pollen germination and tube growth when they were in lower mass concentrations,but they would inhibit pollen germination and tube growth when they were in higher mass concentrations.[Concluson] This research will provide the fundamental data for further studying physiological properties of Solanum tuberosum L..