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Screening high-efficient biocontrol bacterium of Bacillus subtilis by ion implantation and its application against postharvest anthracnose on apple fruit

LIU Shu-fang,TAN Gen-jia,LI Na,HOU Xiao-dan,LI Zeng-zhi(School of Plant Protection,Anhui Agricultural University,Hefei 230036)  
An antagonistic strain Bacillus subtilis produces large amount of antifungal substance with strong inhibiting activity against plant fungi.We obtained mutants by the technology of low energy ion and screened out 6 mutant isolates which can effectively control postharvest anthracnose of apple fruits by plate test.From which,two strains(BS 80-6,BS100-6) showed significant control effect in vivo under room temperature and low temperature,and their control effects were 33.28%and 100% respectively in the fourteenth day.
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