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Gene expression profiles of toxicated BALB/c mouse liver exposed to tetracycline

GAO Li-hong~(1),AO Lin~(1),HU Ran~(1),LIU Jin-yi~(1),HUANG Ming-hui~(2),YANG Meng-su~(2),CAO Jia~(1)(~(1)Department of Hygiene Toxicology,College of Preventive Medicine,Third Military Medical University,Chongqing 400038,~(2)Genome Technological Application Institute,City University of Hong Kong)  
Objective To study the molecular mechanism of the toxic effect for tetracycline acting on liver using toxicological microarray.Methods Utilizing the mouse toxicological microarray and animal model of tetracycline injuring BALB/c mouse liver,both of which were established by our department,we observed the gene expression profiles in different dose groups and at different time points after tetracycline treatment,and primarily analysed the function of these differentially expressed genes using Gene Ontology Consortium analysis system and hierarchical method.Results Multiple differentially expressed genes were found,and an obvious difference in the profiles was found in those treated with high-dose and low-dose tetracycline.The differentially expressed genes of all time points in high-dose group were divided to four clusters,which respectively related to the molecular mechanism of repressed enegy metabolism,enhanced protein synthesis and degradation,impared system of resisting oxidation,signal transduction genes changing to accelerate apoptosis,repressed genes associated of drug metabolism.Conclusion The analysis using toxicological microarray can offer us many clues on molecular level for studying the mechanism of the toxic effect of tetracycline acting on liver.
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