Construction of Vector for Oilseed Rape Chloroplast Transformation and Its Insecticidal Toxicity
Hou Bingkai, Zhang Zhonglin , Zhou Yihua Zhang Yinmei, Shen Guifang , Chen Zhenghua (Institute of Genetics, Chinese Academy of Sciences, Beijing 100101) ( Biotechnology Research institute, Chinese Academy of Agricultural Sciences, Beijing
WT5BZ]1.0 kb and 2.4 kb DNA fragment containing rps7 gene and ndhB gene, respectively, were cloned from oilseed rape chloroplast genome. A full length 3.5 kb Bt toxin gene ( cry1Aa ) was also cloned from the plasmid of Bacillus thuringiensis. The vector containing Bt toxin gene and aadA gene for oilseed rape chloroplast transformation was constructed with the rps7 gene and ndhB gene as homologous recombinant fragments. Bioassays using crude expressed products from host strain of the vector showed that Bt toxin gene was expressed and its products were toxic to larvae of Heliothis armigera. The chloroplast transformation vector will be of great value to oilseed rape chloroplast transformation and to the insect resistant crop improvement.