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Target genes of homo sapiens microRNA-142-5p and its association with cardiovascular diseases: a bioinformatics-based analysis

YU Hong-yu;ZHOU Shuang;JIANG Zhi-yuan;Department of Cardiovascular Medicine, the First Affiliated Hospital of Guangxi Medical University;  
Objective To predict the target genes of homo sapiens microRNA-142-5p(hsa-miR-142-5p) using bioinformatics technology, and to preliminarily investigate the role of has-miR-142-5p in cardiovascular diseases by analyzing the biological processes and signaling pathways which the target genes involved. Methods The expression of has-miR-142-5p in various tissues and organs was searched in the miRGator v3.0 database. The general information about the localization, base sequence, and species conservative property of has-miR-142-5p was obtained by combining the hsa-miR-142-5 p-related literature collected from the PubMed database with the results of the retrieval on the miRBase 22.1 and UCSC Genome Browser. The target genes were predicted using the databases such as TargetScan, DIANA TOOLS, miRDB, and miRWalk followed by the intersection, which were merged with the target genes verified experimentally in the miRTarBase database to serve as a gene set. Gene Ontology(GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genome(KEGG) pathway enrichment analysis were performed on the gene set enrolled. Results The hsa-miR-142-5p was basically expressed in the heart and was highly conserved in 26 different species including homo sapiens. A target gene set comprising 51 candidate genes was obtained. The target genes were functionally enriched in the biological processes associated with the cardiovascular system, including endocardial cushion fusion, embryonic angiopoiesis and development, ventricular morphogenesis, regulation of cardiac hypertrophy, and positive regulation of cardiac epithelial-to-mesenchymal transition(P0.05), and were involved in the regulation of signaling pathways such as phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt) signaling pathway, adhesion junctions, transforming growth factor β(TGF-β) signaling pathway, thyroid hormone signaling pathway, Rap1 signaling pathway, and forkhead box O(FoxO) signaling pathway(all P0.05). Conclusion The target genes of hsa-miR-142-5p may be associated with congenital heart disease, cardiac hypertrophy, myocardial fibrosis, and the regulation of vascular smooth muscle cells and vascular endothelial cells; moreover, PI3K/Akt signaling pathway, adhesion junctions, TGF-β signaling pathway, thyroid hormone signaling pathway, Rap1 signaling pathway, and FoxO signaling pathway may be involved in the aforementioned pathophysiology.
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