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Effects of Sishen pill on expression of type Ⅰ and type Ⅲ interferon in acute ulcerative colitis mice model

DONG Jia-min;LU Tao;LI Ke;LI Meng-jia;WANG Xu-dan;GE Dong-yu;WU Ying;Beijing University of Chinese Medicine;Liuzhou People's Hospital Affiliated to Guangxi Medical University;  
Objective:To investigate the effects of Sishen pill on the expression of typeⅠinterferon and type Ⅲ interferon and their receptors in colonic tissues of mice with acute ulcerative colitis. Methods:Male C57 BL/6 Cnc mice were randomly divided into control group,model group,sishen pill group and salazosulfapyridine group. The models were induced with 0.2 mL 4%dextran sodium sulfate(DSS)for 5 days,and the control group was given 0.2 mL normal saline by gavage. On the second day of modeling,sishen pill group was given 0.2 mL 1.5 g/kg sishen pill,and SASP group was given 0.2 mL 0.25 g/kg sulfasalazine twice a day for 7 days. During the administration period,the disease activity index(DAI)of mice was calculated every day. After administration,the histopathological changes of colon tissues of mice in each group were observed by hematoxylin eosin(HE)staining,and the histological scores were calculated. The expression of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 mRNAs in colon tissues of mice in each group were detected by qRT-PCR. The expression levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 in colon tissues of mice in each group were detected by ELISA. Western blot was used to detect the expression of interferon receptors IFNAR1,IFNAR2 and IFNLR1 in colon tissues of mice in each group. Results:Compared with the control group,the DAI of mice increased significantly(P0.001)in the model group. The inflammatory cells in colonic tissues infiltrated heavily,lymph nodes enlarged,colonic mucosal structure destroyed,crypt structure lost,inflammation involved a wide range,and the histological score increased significantly(P0.001). The levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 mRNA were significantly decreased(P0.01,P0.05,P0.05 and P0.05,respectively). The expression levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 were significantly decreased(P0.01,P0.01,P0.001 and P0.001,respectively). The levels of IFNAR1,IFNAR2 and IFNLR1 were significantly decreased(P0.01,P0.05 and P0.01,respectively). Compared with the model group,the DAI decreased significantly(P0.001)in Sishen pill group,the infiltration of inflammatory cells in colon tissue were significantly reduced,the structural regeneration of colon mucosa was significantly recovered,the crypt structure was significantly recovered,the lymph nodes were significantly reduced,the range of inflammation involvement was reduced,and the histological score was significantly reduced(P0.001). The levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 mRNA were significantly increased(P0.001,P0.01,P0.001 and P0.01,respectively). The levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 were significantly increased(P0.01,P0.001,P0.01 and P0.001,respectively). The levels of IFNAR1,IFNAR2 and IFNLR1 were significantly increased(P0.05,P0.001 and P0.05,respectively). Conclusion:Sishen pill may alleviate the symptoms and signs of mice with acute ulcerative colitis by regulating the expression of typeⅠand type Ⅲinterferon and their receptors in colon tissues.
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