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A Preliminary Study on the Anti-tumor Activity of Alcohol Extracts of Pini Pollen

LI Wen-wen①,CHEN Cai-fa②,LI Yue③,ZHU Yu-jie①①College of Biological Science,②Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu province,Xuzhou Normal University,Xuzhou221116,China;③No.2 Attached Elementary School,Jiangsu China Lianyungang Teachers College,Lianyungang222023,China  
Objective To investigate the anti-tumor activities of alcohol extracts of Pini Pollen in vivo and in vitro.Methods In vitro test:the MTT method was used to determine the inhibitory effect of alcohol extracts of Pollen Pini on 247,HCT116,HeLa and K293.In vivo test:mice with Lewis lung carcinoma were used in this study,The weight of the tumor,thymus,spleen,the tumor inhibitory rate and immune organ index in each group were calculated.Results Alcohol extracts of Pollen Pini had obvious cytotoxicity to various tumor cells but less cytotoxicity to normal cell K293,Capable of significant inhibition on the growth of Lewis lung carcinoma transplanted tumor and can increase the thymus and spleen index of mice.Conclusion Alcohol extracts of Pollen Pini can effectively inhibit tumor and protect immune organs.
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