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Establishment of a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice

ZHOU Qifeng;ZHANG Lingqiang;LIAO Mengjiao;Medical College of Qinghai University;  
Objective To establish a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice,and to lay a foundation for further research on alveolar echinococcosis. Methods A total of 25 nude mice were given subcutaneous injection of 0. 1 ml 20% Echinococcus multilocularis protoscolex suspension at the back of the neck. The growth of lesions was observed and recorded,and trypan blue staining was performed at 6 months after injection to observe the cell viability of protoscolices. Results Subcutaneous lesions were observed at about 8 days after injection. At 3 months after the establishment of the model,the survival rate of the nude mice was 92%( 23/25),and the infection rate of Echinococcus multilocularis was 100%( 23/23). A small number of protoscolices died and were blue on trypan blue staining,and the cell viability of protoscolices was 98%. Conclusion Echinococcus multilocularis can be successfully transplanted to the subcutaneous part of nude mice,which provides a reference for further studies on the establishment of animal models of alveolar echinococcosis.
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