Research on the mass concentration and antioxidation of proanthocyanidins in different tissues from Phyllanthus emblica Linn.
ZHANG Lun;YANG Shenming;XU Chengdong;WANG Fei;HU Xiaoan;College of Chemistry and Life Science,Chuxiong Normal University;Nanjing Forestry University;
Proanthocyanidins are one of the most effective free radical scavenger,which have strong capability of scavenging free radical and antioxidation. Taking the Phyllanthus emblica Linn. tree in Yunan Provnice as the rawmaterial,the content of proanthocyanidins in barks,tree cores,branches and leaves were determined by ultrasonic extraction technology. In the same time,the extraction conditions were optimized. In additon,antioxidant activities and VC( vitamin C) synergistic effect of proanthocyanidins from Ph. emblica Linn. barks were compared. The results showed that the content of proanthocyanidins is the highest in the branch; followed by the barks,the leaves,and the tree core.The optimum extraction conditions of proanthocyanidins from the branch were as follows: with 60% ethanol as the extracting agent,solid-liquid ratio of 1 ∶7,temperature of 60℃ and time for 30 min. Under this best condition,the extraction rate could reach 3. 85%. The extraction has strong antioxidant activity from Ph. emblica barks. Scavenging DPPH ability of free radical was the best when proanthocyanidin concentration was 10 μg/m L,the highest scavenging rate was 88. 55%. Antioxidant active ingredients were mainly concentrated in the ethyl acetate layer and the aqueous layer also contains part of the antioxidants. The scavenging rates were 88. 84%,91. 27% and 91. 05%,respectively,when the complex concentrations of proanthocyanidins and VC was 3 ∶1,1 ∶1 and 1 ∶3,respectively,and the effects of synergy showed the result of 1 ∶11 ∶33 ∶1.