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Extraction and Purification of Phosphatidylcholine from Egg Yolk

XU Xiao-hui1,ZHAO Bin-xia2,ZHAO Li-xin1(1.The Technology Center of Zibo Mining Group Ltd.,Zibo 255120,China;2.Department of Chemical Engineering,Northwest University,Xi′an 710069,China)  
With egg yolk as raw material,extracted with aqueous ethanol and ether,and removing water and oil with acetone,a crude phospholipids mixture was obtained.Then the thin-layer chromatography was used with column on silicic acid to purify the phospholipids mixture.Eluated with the solvent mixture(choroform/methanol),ultimately a highly pure white lecithin was obtained.Analysised by thin layer chromatography,there is only one spot.With thin-layer chromatography-scanning method to assay the purity it is higher than 97.4%.
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